Impaired microglia fractalkine signaling affects stress reaction and coping style in mice

Zsuzsanna Winkler, Dániel Kuti, Szilamér Ferenczi, Krisztina Gulyás, Ágnes Polyák, K. Kovács

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Microglia, resident immune cells of the CNS are sensitive to various perturbations of the environment, such as stress exposure, and may be involved in translating these changes to behavior. Among the pathways mediating stress-related neuronal cues to microglia, the fractalkine-fractalkine receptor (CX3CR1) signaling plays a crucial role. Using mice, in which the CX3CR1 gene was deleted, we explored hormonal and behavioral responses to acute and chronic stress along with changes in hypothalamic microglia. CX3CR1−/− animals display active escape in forced swim- and tail suspension tests, exaggerated neuronal activation in the hypothalamic paraventricular nucleus and increased corticosterone release in response to restraint. Analysis of Iba1 immunostaining of hypothalamic sections revealed stress-related reduction of microglia in CX3CR1−/− mice. Because microglia also contribute to energy balance regulation, we characterized metabolic phenotype of CX3CR1−/− mice. Comparison of respiratory exchange ratio did not show genotype effect on fuel preference, however, the energy expenditure was increased in CX3CR1−/− mice, which may be related to their active coping behavior. Microglia and fractalkine signaling has been repeatedly shown to be involved chronic stress-induced depressive state. CX3CR1−/− mice did not become anhedonic in the “two hit” chronic stress paradigm, confirming resistance of these animals to chronic stress-induced mood alterations. However, there was no difference in stress hormone levels, open field performance and hypothalamic microglia distribution between the genotypes. These results highlight differential involvement of microglia fractalkine signaling in controlling/integrating hormonal-, metabolic and behavioral responses to acute and chronic stress challenges.

Original languageEnglish
Pages (from-to)119-128
Number of pages10
JournalBehavioural Brain Research
Volume334
DOIs
Publication statusPublished - Sep 15 2017

Keywords

  • c-Fos
  • Chronic stress
  • CXCR1
  • Depression
  • Energy expenditure
  • Microglia

ASJC Scopus subject areas

  • Behavioral Neuroscience

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