Impaired metabolism in donor kidney grafts after steroid pretreatment

Julia Wilflingseder, Alexander Kainz, Irmgard Mühlberger, Paul Perco, R. Langer, Ivan Kristo, Bernd Mayer, Rainer Oberbauer

Research output: Contribution to journalArticle

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Abstract

We recently showed in a randomized control trial that steroid pretreatment of the deceased organ donor suppressed inflammation in the transplant organ but did not reduce the rate or duration of delayed graft function (DGF). This study sought to elucidate such of those factors that caused DGF in the steroid-treated subjects. Genome-wide gene expression profiles were used from 20 steroid-pretreated donor-organs and were analyzed on the level of regulatory protein-protein interaction networks. Significance analysis of microarrays (SAM) yielded 63 significantly down-regulated sequences associated with DGF that could be functionally categorized according to Protein ANalysis THrough Evolutionary Relationships ontologies into two main biologic processes: transport (P <0.001) and metabolism (P <0.001). The identified genes suggest hypoxia as the cause of DGF, which cannot be counterbalanced by steroid treatment. Our data showed that molecular pathways affected by ischemia such as transport and metabolism are associated with DGF. Potential interventional targeted therapy based on these findings includes peroxisome proliferator-activated receptor agonists or caspase inhibitors.

Original languageEnglish
Pages (from-to)796-804
Number of pages9
JournalTransplant International
Volume23
Issue number8
DOIs
Publication statusPublished - 2010

Fingerprint

Delayed Graft Function
Steroids
Tissue Donors
Transplants
Kidney
Biological Transport
Protein Interaction Maps
Peroxisome Proliferator-Activated Receptors
Caspase Inhibitors
Microarray Analysis
Transcriptome
Proteins
Ischemia
Genome
Inflammation
Therapeutics
Genes

Keywords

  • bioinformatics
  • delayed graft function
  • renal transplantation
  • system biology
  • transcriptome

ASJC Scopus subject areas

  • Transplantation
  • Medicine(all)

Cite this

Wilflingseder, J., Kainz, A., Mühlberger, I., Perco, P., Langer, R., Kristo, I., ... Oberbauer, R. (2010). Impaired metabolism in donor kidney grafts after steroid pretreatment. Transplant International, 23(8), 796-804. https://doi.org/10.1111/j.1432-2277.2010.01053.x

Impaired metabolism in donor kidney grafts after steroid pretreatment. / Wilflingseder, Julia; Kainz, Alexander; Mühlberger, Irmgard; Perco, Paul; Langer, R.; Kristo, Ivan; Mayer, Bernd; Oberbauer, Rainer.

In: Transplant International, Vol. 23, No. 8, 2010, p. 796-804.

Research output: Contribution to journalArticle

Wilflingseder, J, Kainz, A, Mühlberger, I, Perco, P, Langer, R, Kristo, I, Mayer, B & Oberbauer, R 2010, 'Impaired metabolism in donor kidney grafts after steroid pretreatment', Transplant International, vol. 23, no. 8, pp. 796-804. https://doi.org/10.1111/j.1432-2277.2010.01053.x
Wilflingseder, Julia ; Kainz, Alexander ; Mühlberger, Irmgard ; Perco, Paul ; Langer, R. ; Kristo, Ivan ; Mayer, Bernd ; Oberbauer, Rainer. / Impaired metabolism in donor kidney grafts after steroid pretreatment. In: Transplant International. 2010 ; Vol. 23, No. 8. pp. 796-804.
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