Impaired humoral immune response against mycobacterial 65-kDa heat shock protein (HSP65) in patients with inflammatory bowel disease

L. Bene, G. Füst, Zoltán Huszti, Zsolt Hernádi, B. Fekete, Márta Mészáros, A. Veres, A. Kovács, Kata Miklós, Mahavir Singh, László Romics, Z. Prohászka

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Since only scarce data are available on the immune response against heat shock proteins (HSP) in inflammatory bowel disease (IBD), we have measured with an ELISA method serum levels of IgG, IgA, and IgM antibodies to mycobacterial HSP65 and human HSP60 in 66 patients with Crohn's disease (CD), 42 patients with ulcerative colitis (UC), and 126 age- and gender-matched healthy controls. Serum concentration [median (25th-75th percentiles) of IgG anti-HSP65 antibodies was substantially lower in patients with either CD (P <0.01) or UC (P <0.001) than in healthy controls, while no difference was found in the levels of anti-HSP60 antibodies. Low anti-HSP65 antibody levels were measured in patients with active CD and in both active and inactive UC, and only in IBD patients with no extraintestinal manifestations. In conclusion, our present findings indicate that an abnormal immune response to bacterial HSP65 or some epitopes of the protein may contribute to the dysregulation of host defenses against certain intestinal bacteria.

Original languageEnglish
Pages (from-to)1432-1437
Number of pages6
JournalDigestive Diseases and Sciences
Volume47
Issue number7
DOIs
Publication statusPublished - 2002

Fingerprint

Humoral Immunity
Heat-Shock Proteins
Inflammatory Bowel Diseases
Ulcerative Colitis
Crohn Disease
Anti-Idiotypic Antibodies
Immunoglobulin G
Serum
Immunoglobulin A
Immunoglobulin M
Epitopes
Enzyme-Linked Immunosorbent Assay
Bacteria
Antibodies
Proteins

Keywords

  • Crohn's disease
  • Heat shock protein
  • HSP60
  • HSP65
  • IBD
  • Ulcerative colitis

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Impaired humoral immune response against mycobacterial 65-kDa heat shock protein (HSP65) in patients with inflammatory bowel disease. / Bene, L.; Füst, G.; Huszti, Zoltán; Hernádi, Zsolt; Fekete, B.; Mészáros, Márta; Veres, A.; Kovács, A.; Miklós, Kata; Singh, Mahavir; Romics, László; Prohászka, Z.

In: Digestive Diseases and Sciences, Vol. 47, No. 7, 2002, p. 1432-1437.

Research output: Contribution to journalArticle

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AU - Romics, László

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