Impaired function of innate T lymphocytes and NK cells in the acute phase of ischemic stroke

Agnes Peterfalvi, Tihamer Molnar, Miklos Banati, Gabriella Pusch, Eva Miko, L. Bogár, J. Pál, L. Szereday, Zsolt Illes

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background: Functional alterations of innate lymphocytes, which can mount rapid immune responses and shape subsequent T cell reactions, were examined in the acute phase of ischemic stroke. Methods: Frequencies, intracellular perforin and interferon-γ (IFN-γ) expression of Vδ2 T cells, CD3+ CD56+ natural killer T (NKT)-like and NK cells were examined in the peripheral blood of 20 healthy controls and 28 patients within 6 h of the onset of acute ischemic stroke and after 72 h by flow cytometry. Cytokine production of isolated NKT-like and NK cells following in vitro activation was measured by cytometric bead array. NK cytotoxicity was examined in the peripheral blood mononuclear cells. Results: Percentages of Vδ2, NKT-like and NK cells were constant, and similar to percentages in healthy subjects. In contrast, proinflammatory intracellularIFN-γ expression by Vδ2 T cells, NKT-like cells and NK cells and IFN-γ production by isolated NK cells in culture was low at 6 h and reached the level of healthy subjects by 72 h after stroke. Production of anti-inflammatory cytokines was unaltered. Intracellular perforin expression by Vδ2 T cells, NKT-like cells and NK cells, and NK cytotoxicity was low at 6 h, and reached the level of healthy subjects by 72 h. Increases in IFN-γ and perforin expression by Vδ2 T cells correlated with clinical improvement indicated by decreases in NIHSS scores. Conclusions: Pro-inflammatory and cytotoxic responses of NK, NKT-like and Vδ2 T cells become acutely deficient in ischemic stroke, which may contribute to an increased susceptibility to infections.

Original languageEnglish
Pages (from-to)490-498
Number of pages9
JournalCerebrovascular Diseases
Volume28
Issue number5
DOIs
Publication statusPublished - Oct 2009

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Natural Killer T-Cells
Natural Killer Cells
Stroke
T-Lymphocytes
Perforin
Interferons
Healthy Volunteers
Cytokines
Blood Cells
Flow Cytometry
Anti-Inflammatory Agents
Cell Culture Techniques
Lymphocytes
Infection

Keywords

  • γδ T cells
  • Innate immunity
  • NK cells
  • NKT cells
  • Stroke

ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

Impaired function of innate T lymphocytes and NK cells in the acute phase of ischemic stroke. / Peterfalvi, Agnes; Molnar, Tihamer; Banati, Miklos; Pusch, Gabriella; Miko, Eva; Bogár, L.; Pál, J.; Szereday, L.; Illes, Zsolt.

In: Cerebrovascular Diseases, Vol. 28, No. 5, 10.2009, p. 490-498.

Research output: Contribution to journalArticle

Peterfalvi, Agnes ; Molnar, Tihamer ; Banati, Miklos ; Pusch, Gabriella ; Miko, Eva ; Bogár, L. ; Pál, J. ; Szereday, L. ; Illes, Zsolt. / Impaired function of innate T lymphocytes and NK cells in the acute phase of ischemic stroke. In: Cerebrovascular Diseases. 2009 ; Vol. 28, No. 5. pp. 490-498.
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