Impact of the reactive microenvironment on the bone marrow involvement of follicular lymphoma

Hajnalka Rajnai, Csaba Bödör, Zsófia Balogh, Éva Gagyi, Judit Csomor, T. Krenács, Erika Tóth, A. Matolcsy

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Aims: Follicular lymphoma (FL) is associated with bone marrow (BM) involvement in approximately 40-70% of cases. Previous studies have suggested that the immune-microenvironment of FL plays an important role in the clinical behaviour of the disease. To investigate the role of the microenvironment in BM involvement of FL, we performed immunophenotypical analysis of the reactive cell populations in lymph nodes (LN) and corresponding BM of 35 patients with FL. Microenvironment patterns of BM infiltrates were compared to the corresponding features of the LN in cases with BM manifestation, and the LN microenvironment was compared in FL cases with and without BM involvement. Methods and results: Automated image-segmentation-based quantitation was performed in whole digital slides of tissue microarrays of formalin-fixed paraffin-embedded tissue biopsies. We found significantly more CD8+ T lymphocytes, forkhead box protein 3 (FoxP3)+ T lymphocytes and CD68+ macrophages and fewer PD1+ T lymphocytes in the BM than in the matching LN samples. Furthermore, we observed significantly fewer CD8+ T cells and CD68+ macrophages in cases involving the BM compared to those localized only to the LNs. Conclusions: Our study showed that different tumour cell growth in the LN and BM may generate different microenvironments, and suggested that the reduced number of cytotoxic T lymphocytes and macrophages in LNs favours BM infiltration of neoplastic cells in FL.

Original languageEnglish
JournalHistopathology
Volume60
Issue number6 B
DOIs
Publication statusPublished - May 2012

Fingerprint

Follicular Lymphoma
Bone Marrow
Lymph Nodes
T-Lymphocytes
Macrophages
Forkhead Transcription Factors
Cytotoxic T-Lymphocytes
Paraffin
Formaldehyde
Biopsy

Keywords

  • Automated image-segmentation-based quantitation
  • Bone marrow
  • Follicular lymphoma
  • Microenvironment
  • Tissue microarray

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine

Cite this

Impact of the reactive microenvironment on the bone marrow involvement of follicular lymphoma. / Rajnai, Hajnalka; Bödör, Csaba; Balogh, Zsófia; Gagyi, Éva; Csomor, Judit; Krenács, T.; Tóth, Erika; Matolcsy, A.

In: Histopathology, Vol. 60, No. 6 B, 05.2012.

Research output: Contribution to journalArticle

Rajnai, Hajnalka ; Bödör, Csaba ; Balogh, Zsófia ; Gagyi, Éva ; Csomor, Judit ; Krenács, T. ; Tóth, Erika ; Matolcsy, A. / Impact of the reactive microenvironment on the bone marrow involvement of follicular lymphoma. In: Histopathology. 2012 ; Vol. 60, No. 6 B.
@article{ab330f2a87d4491f8ed4a2f98f04b93e,
title = "Impact of the reactive microenvironment on the bone marrow involvement of follicular lymphoma",
abstract = "Aims: Follicular lymphoma (FL) is associated with bone marrow (BM) involvement in approximately 40-70{\%} of cases. Previous studies have suggested that the immune-microenvironment of FL plays an important role in the clinical behaviour of the disease. To investigate the role of the microenvironment in BM involvement of FL, we performed immunophenotypical analysis of the reactive cell populations in lymph nodes (LN) and corresponding BM of 35 patients with FL. Microenvironment patterns of BM infiltrates were compared to the corresponding features of the LN in cases with BM manifestation, and the LN microenvironment was compared in FL cases with and without BM involvement. Methods and results: Automated image-segmentation-based quantitation was performed in whole digital slides of tissue microarrays of formalin-fixed paraffin-embedded tissue biopsies. We found significantly more CD8+ T lymphocytes, forkhead box protein 3 (FoxP3)+ T lymphocytes and CD68+ macrophages and fewer PD1+ T lymphocytes in the BM than in the matching LN samples. Furthermore, we observed significantly fewer CD8+ T cells and CD68+ macrophages in cases involving the BM compared to those localized only to the LNs. Conclusions: Our study showed that different tumour cell growth in the LN and BM may generate different microenvironments, and suggested that the reduced number of cytotoxic T lymphocytes and macrophages in LNs favours BM infiltration of neoplastic cells in FL.",
keywords = "Automated image-segmentation-based quantitation, Bone marrow, Follicular lymphoma, Microenvironment, Tissue microarray",
author = "Hajnalka Rajnai and Csaba B{\"o}d{\"o}r and Zs{\'o}fia Balogh and {\'E}va Gagyi and Judit Csomor and T. Kren{\'a}cs and Erika T{\'o}th and A. Matolcsy",
year = "2012",
month = "5",
doi = "10.1111/j.1365-2559.2012.04187.x",
language = "English",
volume = "60",
journal = "Histopathology",
issn = "0309-0167",
publisher = "Wiley-Blackwell",
number = "6 B",

}

TY - JOUR

T1 - Impact of the reactive microenvironment on the bone marrow involvement of follicular lymphoma

AU - Rajnai, Hajnalka

AU - Bödör, Csaba

AU - Balogh, Zsófia

AU - Gagyi, Éva

AU - Csomor, Judit

AU - Krenács, T.

AU - Tóth, Erika

AU - Matolcsy, A.

PY - 2012/5

Y1 - 2012/5

N2 - Aims: Follicular lymphoma (FL) is associated with bone marrow (BM) involvement in approximately 40-70% of cases. Previous studies have suggested that the immune-microenvironment of FL plays an important role in the clinical behaviour of the disease. To investigate the role of the microenvironment in BM involvement of FL, we performed immunophenotypical analysis of the reactive cell populations in lymph nodes (LN) and corresponding BM of 35 patients with FL. Microenvironment patterns of BM infiltrates were compared to the corresponding features of the LN in cases with BM manifestation, and the LN microenvironment was compared in FL cases with and without BM involvement. Methods and results: Automated image-segmentation-based quantitation was performed in whole digital slides of tissue microarrays of formalin-fixed paraffin-embedded tissue biopsies. We found significantly more CD8+ T lymphocytes, forkhead box protein 3 (FoxP3)+ T lymphocytes and CD68+ macrophages and fewer PD1+ T lymphocytes in the BM than in the matching LN samples. Furthermore, we observed significantly fewer CD8+ T cells and CD68+ macrophages in cases involving the BM compared to those localized only to the LNs. Conclusions: Our study showed that different tumour cell growth in the LN and BM may generate different microenvironments, and suggested that the reduced number of cytotoxic T lymphocytes and macrophages in LNs favours BM infiltration of neoplastic cells in FL.

AB - Aims: Follicular lymphoma (FL) is associated with bone marrow (BM) involvement in approximately 40-70% of cases. Previous studies have suggested that the immune-microenvironment of FL plays an important role in the clinical behaviour of the disease. To investigate the role of the microenvironment in BM involvement of FL, we performed immunophenotypical analysis of the reactive cell populations in lymph nodes (LN) and corresponding BM of 35 patients with FL. Microenvironment patterns of BM infiltrates were compared to the corresponding features of the LN in cases with BM manifestation, and the LN microenvironment was compared in FL cases with and without BM involvement. Methods and results: Automated image-segmentation-based quantitation was performed in whole digital slides of tissue microarrays of formalin-fixed paraffin-embedded tissue biopsies. We found significantly more CD8+ T lymphocytes, forkhead box protein 3 (FoxP3)+ T lymphocytes and CD68+ macrophages and fewer PD1+ T lymphocytes in the BM than in the matching LN samples. Furthermore, we observed significantly fewer CD8+ T cells and CD68+ macrophages in cases involving the BM compared to those localized only to the LNs. Conclusions: Our study showed that different tumour cell growth in the LN and BM may generate different microenvironments, and suggested that the reduced number of cytotoxic T lymphocytes and macrophages in LNs favours BM infiltration of neoplastic cells in FL.

KW - Automated image-segmentation-based quantitation

KW - Bone marrow

KW - Follicular lymphoma

KW - Microenvironment

KW - Tissue microarray

UR - http://www.scopus.com/inward/record.url?scp=84860347823&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84860347823&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2559.2012.04187.x

DO - 10.1111/j.1365-2559.2012.04187.x

M3 - Article

C2 - 22394030

AN - SCOPUS:84860347823

VL - 60

JO - Histopathology

JF - Histopathology

SN - 0309-0167

IS - 6 B

ER -