Impact of repeated bouts of eccentric exercise on myogenic gene expression

Andreas Costa, Hicham Dalloul, H. Hegyesi, Peter Apor, Zsolt Csende, Levente Racz, Mark Vaczi, J. Tihanyi

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Evidence indicates that repeated-bouts of eccentric exercise (EE) do not exacerbate the extent of muscle damage indices, as compared to a single-bout. We hypothesized that molecular adaptations, under repeated-bouts of EE, would include suppression of muscle repair inhibitory factors such as myostatin and up-regulation of muscle repair positive regulatory factors such as myogenic regulatory factors (MRFs). Fifteen males were recruited for this study. The exercise group (n = 9) successfully completed six sets of 15 reps of maximum voluntary eccentric contractions, for six consecutive days, using a dynamometer (Multicont-II). Blood and muscle biopsy samples were obtained from each subject 1 week prior to exercise, 2 days post the first training session, and 24 h after the last training session. Gene expression levels were determined using real-time RT-PCR. Blood samples were analyzed for creatine kinase (CK) and lactate-dehydrogenase (LDH) activity. Repeated-bouts of EE induced a large down-regulation of myostatin mRNA (-73%) which persisted throughout the study. The responses of MRFs were mild. At day 3 only myogenin increased significantly (1.9 fold) while MyoD decreased by 45%. Surprisingly, at day 7, despite the presence of muscle damage indices, all MRFs returned to the pre-exercise levels. The results of the present study showed that repeated-bouts of EE, for six consecutive days, dramatically decreased Myostatin mRNA expression but impaired the expression patterns of MRFs such that, with the exception of myogenin that showed a moderate non-sustained increase, MyoD and MYf5 response was minimal.

Original languageEnglish
Pages (from-to)427-436
Number of pages10
JournalEuropean Journal of Applied Physiology
Volume101
Issue number4
DOIs
Publication statusPublished - Nov 2007

Fingerprint

Myogenic Regulatory Factors
Myostatin
Gene Expression
Muscles
Myogenin
Messenger RNA
Creatine Kinase
L-Lactate Dehydrogenase
Real-Time Polymerase Chain Reaction
Up-Regulation
Down-Regulation
Biopsy

Keywords

  • Ki-67
  • Muscle damage
  • Myogenic regulatory factors
  • Myostatin
  • p21

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Impact of repeated bouts of eccentric exercise on myogenic gene expression. / Costa, Andreas; Dalloul, Hicham; Hegyesi, H.; Apor, Peter; Csende, Zsolt; Racz, Levente; Vaczi, Mark; Tihanyi, J.

In: European Journal of Applied Physiology, Vol. 101, No. 4, 11.2007, p. 427-436.

Research output: Contribution to journalArticle

Costa, Andreas ; Dalloul, Hicham ; Hegyesi, H. ; Apor, Peter ; Csende, Zsolt ; Racz, Levente ; Vaczi, Mark ; Tihanyi, J. / Impact of repeated bouts of eccentric exercise on myogenic gene expression. In: European Journal of Applied Physiology. 2007 ; Vol. 101, No. 4. pp. 427-436.
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