Impact of phase transition on the mouse oocyte spindle during vitrification

Ching Chien Chang, Chih Jen Lin, Li Ying Sung, Hilton I. Kort, X. Cindy Tian, P. Nagy

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

During vitrification, the glass-like solidification is the phase-transition process from liquid to solid. Phase transition is one of the major factors suspected to affect the physiology of the oocyte, such as the structure of the meiotic spindle. Therefore, it is very important to investigate the systematic and morphological alterations of the metaphase-II spindle and chromosome arrangement during complete course of a vitrification and warming process. B6D2F1 (C57BL/6 X DBA/2) mouse oocytes were cryopreserved by minimum volume cooling (MVC) method of vitrification in a solution with 15% ethylene glycol, 15% dimethylsulphoxide and 0.5 mol/l sucrose. To examine the spindle, oocytes were fixed before, during and after vitrification and were analysed by immunocytochemistry and confocal microscopy. It was shown that spindles in all oocytes could be maintained through the vitrification and warming process, even though they were exposed to extreme temperature and two rounds of phase transition. According to the sequential observations, chromosome alignment was maintained throughout the complete course of vitrification, warming and post-warming stage. The impact of phase transition was barely detectable when the oocyte was exposed to the vitrification and warming process. The oocyte spindle was able to recover immediately after warming. During oocyte cryopreservation (vitrification), the glass-like solidification is the phase transition process from liquid to solid. Phase transition is one of the major factors suspected to affect the physiology of the oocyte, such as the structure of the meiotic spindle. Therefore, it is very important to investigate the systematic kinetics and morphological alterations of meiotic spindle and chromosome arrangement during the complete course of a vitrification and warming process. To examine the spindle, oocytes were fixed before, during and after vitrification. We showed that spindles in all oocytes could be maintained through the vitrification and warming process, even though they were exposed to extreme temperature and two rounds of phase transition. According to the sequential observations, chromosome alignment was maintained throughout the complete course of vitrification, warming and post-warming stage. The impact of phase transition was barely detectable when the oocyte was exposed to the vitrification and warming process. The oocyte meiotic spindle was able to recover immediately after warming.

Original languageEnglish
Pages (from-to)184-191
Number of pages8
JournalReproductive BioMedicine Online
Volume22
Issue number2
DOIs
Publication statusPublished - Feb 2011

Fingerprint

Vitrification
Phase Transition
Oocytes
Spindle Apparatus
Chromosomes
Glass
Inbred DBA Mouse
Temperature
Ethylene Glycol
Cryopreservation
Metaphase
Dimethyl Sulfoxide
Confocal Microscopy
Sucrose

Keywords

  • meiotic spindle
  • oocyte
  • vitrification

ASJC Scopus subject areas

  • Reproductive Medicine
  • Developmental Biology

Cite this

Impact of phase transition on the mouse oocyte spindle during vitrification. / Chang, Ching Chien; Lin, Chih Jen; Sung, Li Ying; Kort, Hilton I.; Tian, X. Cindy; Nagy, P.

In: Reproductive BioMedicine Online, Vol. 22, No. 2, 02.2011, p. 184-191.

Research output: Contribution to journalArticle

Chang, Ching Chien ; Lin, Chih Jen ; Sung, Li Ying ; Kort, Hilton I. ; Tian, X. Cindy ; Nagy, P. / Impact of phase transition on the mouse oocyte spindle during vitrification. In: Reproductive BioMedicine Online. 2011 ; Vol. 22, No. 2. pp. 184-191.
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