Impact of flavonoid composition of medicinal plants: difficulties in selecting an LC method

V. Vukics, B. Hevesi T, Á Fukász, A. Kéry

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In order to understand the role of flavonoids in the pharmacodynamics of medicinal plants the impact of flavonoid composition on antioxidant activity was studied. Antioxidant capacity was determined by TEAC assay, flavonoid composition was examined by liquid chromatography. This study presents the difficulties experienced in selecting a proper method for analyzing flavonoid aglycones. Standard molecules and plant extracts were examined applying three HPLC systems. System 1 (RP-C18, ACN, CH3COOH-H2O) is similar to the one formerly used for flavonoid glycosides, System 2 (HS-PEG, MeOH-HCOOH, HCOOH-H2O) was recommended by the manufacturer for the very purpose, System 3 (RP-C18, MeOH, H3PO4-H 2O) was developed in our laboratory. Our results show that owing to the complexity of plant extracts, certain identification can only be attained by analyzing the samples in at least three chromatographic systems. Otherwise overlapping of bands may lead to misinterpretation of the chromatograms.

Original languageEnglish
JournalChromatographia
Volume63
Issue numberSUPPL. 13
DOIs
Publication statusPublished - Jun 2006

Fingerprint

Medicinal Plants
Flavonoids
Chemical analysis
Plant Extracts
Antioxidants
Pharmacodynamics
Liquid chromatography
Glycosides
Liquid Chromatography
Polyethylene glycols
Assays
High Pressure Liquid Chromatography
Molecules

Keywords

  • Column liquid chromatography
  • Flavonoid aglycones
  • Plant extracts
  • Qualitative analysis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

Impact of flavonoid composition of medicinal plants : difficulties in selecting an LC method. / Vukics, V.; Hevesi T, B.; Fukász, Á; Kéry, A.

In: Chromatographia, Vol. 63, No. SUPPL. 13, 06.2006.

Research output: Contribution to journalArticle

Vukics, V. ; Hevesi T, B. ; Fukász, Á ; Kéry, A. / Impact of flavonoid composition of medicinal plants : difficulties in selecting an LC method. In: Chromatographia. 2006 ; Vol. 63, No. SUPPL. 13.
@article{42d8c598fb7f4b21b6f6cc0d695a9e2d,
title = "Impact of flavonoid composition of medicinal plants: difficulties in selecting an LC method",
abstract = "In order to understand the role of flavonoids in the pharmacodynamics of medicinal plants the impact of flavonoid composition on antioxidant activity was studied. Antioxidant capacity was determined by TEAC assay, flavonoid composition was examined by liquid chromatography. This study presents the difficulties experienced in selecting a proper method for analyzing flavonoid aglycones. Standard molecules and plant extracts were examined applying three HPLC systems. System 1 (RP-C18, ACN, CH3COOH-H2O) is similar to the one formerly used for flavonoid glycosides, System 2 (HS-PEG, MeOH-HCOOH, HCOOH-H2O) was recommended by the manufacturer for the very purpose, System 3 (RP-C18, MeOH, H3PO4-H 2O) was developed in our laboratory. Our results show that owing to the complexity of plant extracts, certain identification can only be attained by analyzing the samples in at least three chromatographic systems. Otherwise overlapping of bands may lead to misinterpretation of the chromatograms.",
keywords = "Column liquid chromatography, Flavonoid aglycones, Plant extracts, Qualitative analysis",
author = "V. Vukics and {Hevesi T}, B. and {\'A} Fuk{\'a}sz and A. K{\'e}ry",
year = "2006",
month = "6",
doi = "10.1365/s10337-006-0727-8",
language = "English",
volume = "63",
journal = "Chromatographia",
issn = "0009-5893",
publisher = "Springer Vieweg",
number = "SUPPL. 13",

}

TY - JOUR

T1 - Impact of flavonoid composition of medicinal plants

T2 - difficulties in selecting an LC method

AU - Vukics, V.

AU - Hevesi T, B.

AU - Fukász, Á

AU - Kéry, A.

PY - 2006/6

Y1 - 2006/6

N2 - In order to understand the role of flavonoids in the pharmacodynamics of medicinal plants the impact of flavonoid composition on antioxidant activity was studied. Antioxidant capacity was determined by TEAC assay, flavonoid composition was examined by liquid chromatography. This study presents the difficulties experienced in selecting a proper method for analyzing flavonoid aglycones. Standard molecules and plant extracts were examined applying three HPLC systems. System 1 (RP-C18, ACN, CH3COOH-H2O) is similar to the one formerly used for flavonoid glycosides, System 2 (HS-PEG, MeOH-HCOOH, HCOOH-H2O) was recommended by the manufacturer for the very purpose, System 3 (RP-C18, MeOH, H3PO4-H 2O) was developed in our laboratory. Our results show that owing to the complexity of plant extracts, certain identification can only be attained by analyzing the samples in at least three chromatographic systems. Otherwise overlapping of bands may lead to misinterpretation of the chromatograms.

AB - In order to understand the role of flavonoids in the pharmacodynamics of medicinal plants the impact of flavonoid composition on antioxidant activity was studied. Antioxidant capacity was determined by TEAC assay, flavonoid composition was examined by liquid chromatography. This study presents the difficulties experienced in selecting a proper method for analyzing flavonoid aglycones. Standard molecules and plant extracts were examined applying three HPLC systems. System 1 (RP-C18, ACN, CH3COOH-H2O) is similar to the one formerly used for flavonoid glycosides, System 2 (HS-PEG, MeOH-HCOOH, HCOOH-H2O) was recommended by the manufacturer for the very purpose, System 3 (RP-C18, MeOH, H3PO4-H 2O) was developed in our laboratory. Our results show that owing to the complexity of plant extracts, certain identification can only be attained by analyzing the samples in at least three chromatographic systems. Otherwise overlapping of bands may lead to misinterpretation of the chromatograms.

KW - Column liquid chromatography

KW - Flavonoid aglycones

KW - Plant extracts

KW - Qualitative analysis

UR - http://www.scopus.com/inward/record.url?scp=33745174500&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745174500&partnerID=8YFLogxK

U2 - 10.1365/s10337-006-0727-8

DO - 10.1365/s10337-006-0727-8

M3 - Article

AN - SCOPUS:33745174500

VL - 63

JO - Chromatographia

JF - Chromatographia

SN - 0009-5893

IS - SUPPL. 13

ER -