Impact of aging on spreading depolarizations induced by focal brain ischemia in rats

Darren Clark, Ádám Institoris, Gábor Kozák, Zsófia Bere, Ursula Tuor, Eszter Farkas, Ferenc Bari

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19 Citations (Scopus)


Spreading depolarization (SD) contributes to the ischemic damage of the penumbra. Although age is the largest predictor of stroke, no studies have examined age dependence of SD appearance. We characterized the electrophysiological and hemodynamic changes in young (6 weeks old, n= 7), middle-aged (9 months old, n= 6), and old (2 years old, n= 7) male Wistar rats during 30 minutes of middle cerebral artery occlusion (MCAO), utilizing multimodal imaging through a closed cranial window over the ischemic cortex: membrane potential changes (with a voltage-sensitive dye), cerebral blood volume (green light reflectance), and cerebral blood flow (CBF, laser-speckle imaging) were observed. The initial CBF drop was similar in all groups, with a significant further reduction during ischemia in old rats (p < 0.01). Age reduced the total number of SDs (p < 0.05) but increased the size of ischemic area displaying prolonged SD (p < 0.01). The growth of area undergoing prolonged SDs positively correlated with the growth of ischemic core area (p < 0.01) during MCAO. Prolonged SDs and associated hypoperfusion likely compromise cortical tissue exposed to even a short focal ischemia in aged rats.

Original languageEnglish
Pages (from-to)2803-2811
Number of pages9
JournalNeurobiology of Aging
Issue number12
Publication statusPublished - Dec 1 2014



  • Inverse neurovascular coupling
  • Middle cerebral artery occlusion
  • Multimodal imaging
  • Peri-infarct depolarization
  • Voltage-sensitive dye

ASJC Scopus subject areas

  • Neuroscience(all)
  • Ageing
  • Clinical Neurology
  • Developmental Biology
  • Geriatrics and Gerontology

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