Immunotoxicological investigation in rats dosed repeatedly with combinations of cypermethrin, As(III), and Hg(II)

L. Institóris, O. Siroki, Ülkü Ündeger, Nursen Basaran, I. Dési

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The effects of the combined exposure of cypermethrin (CY), NaAsO2 (As), and HgCl2 (Hg) were investigated in male Wistar rats following 4 weeks of oral exposure. Standard toxicological (body weight gain, organ weights of brain, thymus, heart, lung, liver, kidneys, adrenals, testes, and popliteal lymph node), haematological (white blood cell, red blood cell, haematocrit, MCV-mean volume of red blood cells, and cell content of the femoral bone marrow), and immune function (IgM-PFC response, delayed type hypersensitivity (DTH) reaction) parameters were measured. The two doses selected for the combination were: a high (H) dose equal to lowest observed effect level determined in preliminary experiments (CY(H) = 55.4 mg/kg, As(H) = 13.3 mg/kg, Hg(H) = 3.20 mg/kg), and a low (L) dose which was ineffective (non-observed effect level); CY(L) = 11.1 mg/kg, As(L) = 3.33 mg/kg, Hg(L) = 0.40 mg/kg). CY(H) was combined with As(L) or Hg(L), and the H doses of the heavy metals were combined with CY(L). Beside vehicle control, the H dose components of the combinations were also used as internal controls. The main finding of this study was that certain combinations, when compared to H dose internal control, significantly altered the body weight gain (As(H) + CY(L)), the relative weight of adrenals and popliteal lymph node (CY(H) + As(L) and CY(H) + Hg(L)), the cell content of the femoral bone marrow (CY(H) + Hg(L)), and the time course of DTH reaction (CY(H) + As(L)). According to the present results, combined exposure with CY and the heavy metals investigated can modify the toxicity and/or the functional detection limit of the single substances.

Original languageEnglish
Pages (from-to)59-67
Number of pages9
JournalToxicology
Volume172
Issue number1
DOIs
Publication statusPublished - Mar 5 2002

Fingerprint

Mercuric Chloride
Rats
Blood
Cells
Delayed Hypersensitivity
Heavy Metals
Thigh
Weight Gain
Bone
cypermethrin
Erythrocytes
Lymph Nodes
Bone Marrow
Body Weight
Thymus
Organ Size
Hematocrit
Liver
Toxicology
Thymus Gland

Keywords

  • Cypermethrin
  • HgCl
  • Immunotoxicity
  • NaAsO
  • Rats

ASJC Scopus subject areas

  • Toxicology

Cite this

Immunotoxicological investigation in rats dosed repeatedly with combinations of cypermethrin, As(III), and Hg(II). / Institóris, L.; Siroki, O.; Ündeger, Ülkü; Basaran, Nursen; Dési, I.

In: Toxicology, Vol. 172, No. 1, 05.03.2002, p. 59-67.

Research output: Contribution to journalArticle

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abstract = "The effects of the combined exposure of cypermethrin (CY), NaAsO2 (As), and HgCl2 (Hg) were investigated in male Wistar rats following 4 weeks of oral exposure. Standard toxicological (body weight gain, organ weights of brain, thymus, heart, lung, liver, kidneys, adrenals, testes, and popliteal lymph node), haematological (white blood cell, red blood cell, haematocrit, MCV-mean volume of red blood cells, and cell content of the femoral bone marrow), and immune function (IgM-PFC response, delayed type hypersensitivity (DTH) reaction) parameters were measured. The two doses selected for the combination were: a high (H) dose equal to lowest observed effect level determined in preliminary experiments (CY(H) = 55.4 mg/kg, As(H) = 13.3 mg/kg, Hg(H) = 3.20 mg/kg), and a low (L) dose which was ineffective (non-observed effect level); CY(L) = 11.1 mg/kg, As(L) = 3.33 mg/kg, Hg(L) = 0.40 mg/kg). CY(H) was combined with As(L) or Hg(L), and the H doses of the heavy metals were combined with CY(L). Beside vehicle control, the H dose components of the combinations were also used as internal controls. The main finding of this study was that certain combinations, when compared to H dose internal control, significantly altered the body weight gain (As(H) + CY(L)), the relative weight of adrenals and popliteal lymph node (CY(H) + As(L) and CY(H) + Hg(L)), the cell content of the femoral bone marrow (CY(H) + Hg(L)), and the time course of DTH reaction (CY(H) + As(L)). According to the present results, combined exposure with CY and the heavy metals investigated can modify the toxicity and/or the functional detection limit of the single substances.",
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AU - Basaran, Nursen

AU - Dési, I.

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N2 - The effects of the combined exposure of cypermethrin (CY), NaAsO2 (As), and HgCl2 (Hg) were investigated in male Wistar rats following 4 weeks of oral exposure. Standard toxicological (body weight gain, organ weights of brain, thymus, heart, lung, liver, kidneys, adrenals, testes, and popliteal lymph node), haematological (white blood cell, red blood cell, haematocrit, MCV-mean volume of red blood cells, and cell content of the femoral bone marrow), and immune function (IgM-PFC response, delayed type hypersensitivity (DTH) reaction) parameters were measured. The two doses selected for the combination were: a high (H) dose equal to lowest observed effect level determined in preliminary experiments (CY(H) = 55.4 mg/kg, As(H) = 13.3 mg/kg, Hg(H) = 3.20 mg/kg), and a low (L) dose which was ineffective (non-observed effect level); CY(L) = 11.1 mg/kg, As(L) = 3.33 mg/kg, Hg(L) = 0.40 mg/kg). CY(H) was combined with As(L) or Hg(L), and the H doses of the heavy metals were combined with CY(L). Beside vehicle control, the H dose components of the combinations were also used as internal controls. The main finding of this study was that certain combinations, when compared to H dose internal control, significantly altered the body weight gain (As(H) + CY(L)), the relative weight of adrenals and popliteal lymph node (CY(H) + As(L) and CY(H) + Hg(L)), the cell content of the femoral bone marrow (CY(H) + Hg(L)), and the time course of DTH reaction (CY(H) + As(L)). According to the present results, combined exposure with CY and the heavy metals investigated can modify the toxicity and/or the functional detection limit of the single substances.

AB - The effects of the combined exposure of cypermethrin (CY), NaAsO2 (As), and HgCl2 (Hg) were investigated in male Wistar rats following 4 weeks of oral exposure. Standard toxicological (body weight gain, organ weights of brain, thymus, heart, lung, liver, kidneys, adrenals, testes, and popliteal lymph node), haematological (white blood cell, red blood cell, haematocrit, MCV-mean volume of red blood cells, and cell content of the femoral bone marrow), and immune function (IgM-PFC response, delayed type hypersensitivity (DTH) reaction) parameters were measured. The two doses selected for the combination were: a high (H) dose equal to lowest observed effect level determined in preliminary experiments (CY(H) = 55.4 mg/kg, As(H) = 13.3 mg/kg, Hg(H) = 3.20 mg/kg), and a low (L) dose which was ineffective (non-observed effect level); CY(L) = 11.1 mg/kg, As(L) = 3.33 mg/kg, Hg(L) = 0.40 mg/kg). CY(H) was combined with As(L) or Hg(L), and the H doses of the heavy metals were combined with CY(L). Beside vehicle control, the H dose components of the combinations were also used as internal controls. The main finding of this study was that certain combinations, when compared to H dose internal control, significantly altered the body weight gain (As(H) + CY(L)), the relative weight of adrenals and popliteal lymph node (CY(H) + As(L) and CY(H) + Hg(L)), the cell content of the femoral bone marrow (CY(H) + Hg(L)), and the time course of DTH reaction (CY(H) + As(L)). According to the present results, combined exposure with CY and the heavy metals investigated can modify the toxicity and/or the functional detection limit of the single substances.

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