Immunosuppressors inhibit voltage-gated potassium channels in human peripheral blood lymphocytes

G. Panyi, R. Gáspár, Z. Krasznai, Jan J. Ter Horst, Marcel Ameloot, Adorján Aszalós, Paul Steels, S. Damjanovich

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The effects of immunosuppressive agents on the potassium current of human peripheral blood lymphocytes have been studied using the whole-cell patch-clamp technique. Cyclosporin A (10 μg/ml), rapamycin (10 μg/ml) and FK-506 (2.5 μg/ml) reduced the peak K+ current by approximately 40, 30 and 40% of the control, respectively, without any change in the reversal potential of the current. The current inhibition was similar at all membrane potentials studied and was accompanied with an increase in the rate of K+ current inactivation. Membrane potential measurements in current-clamp showed a marked depolarization of the membrane (> 10 mV) upon the addition of either immunosuppressor to the cells. Our findings revealed that the voltage-dependent potassium current in human peripheral blood lymphocytes is inhibited by Cyclosporin A and other immunosuppressors, resulting in a depolarized membrane potential.

Original languageEnglish
Pages (from-to)254-258
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume221
Issue number2
DOIs
Publication statusPublished - Apr 16 1996

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Voltage-Gated Potassium Channels
Lymphocytes
Membrane Potentials
Blood
Membranes
Cyclosporine
Potassium
Clamping devices
Tacrolimus
Patch-Clamp Techniques
Sirolimus
Immunosuppressive Agents
Depolarization
Electric potential

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Immunosuppressors inhibit voltage-gated potassium channels in human peripheral blood lymphocytes. / Panyi, G.; Gáspár, R.; Krasznai, Z.; Ter Horst, Jan J.; Ameloot, Marcel; Aszalós, Adorján; Steels, Paul; Damjanovich, S.

In: Biochemical and Biophysical Research Communications, Vol. 221, No. 2, 16.04.1996, p. 254-258.

Research output: Contribution to journalArticle

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AU - Aszalós, Adorján

AU - Steels, Paul

AU - Damjanovich, S.

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AB - The effects of immunosuppressive agents on the potassium current of human peripheral blood lymphocytes have been studied using the whole-cell patch-clamp technique. Cyclosporin A (10 μg/ml), rapamycin (10 μg/ml) and FK-506 (2.5 μg/ml) reduced the peak K+ current by approximately 40, 30 and 40% of the control, respectively, without any change in the reversal potential of the current. The current inhibition was similar at all membrane potentials studied and was accompanied with an increase in the rate of K+ current inactivation. Membrane potential measurements in current-clamp showed a marked depolarization of the membrane (> 10 mV) upon the addition of either immunosuppressor to the cells. Our findings revealed that the voltage-dependent potassium current in human peripheral blood lymphocytes is inhibited by Cyclosporin A and other immunosuppressors, resulting in a depolarized membrane potential.

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