Immunostaining for substance P receptor labels GABAergic cells with distinct termination patterns in the hippocampus

L. Acsády, I. Katona, A. I. Gulyás, R. Shigemoto, T. Freund

Research output: Contribution to journalArticle

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Abstract

A specific antiserum against substance P receptor (SPR) labels nonprincipal neurons in the cerebral cortex of the rat (T. Kaneko et al. [1994], Neuroscience 60:199-211; Y. Nakaya et al. [1994], J. Comp. Neurol. 347:249-274). In the present study, we aimed to identify the types of SPR- immunoreactive neurons in the hippocampus according to their content of neurochemical markers, which label interneuron populations with distinct termination patterns. Markers for perisomatic inhibitory cells, parvalbumin and cholecystokinin (CCK), colocalized with SPR in pyramidallike basket cells in the dentate gyrus and in large multipolar or bitufted cells within all hippocampal subfields respectively. A dense meshwork of SPR-immunoreactive spiny dendrites in the hilus and stratum lucidum of the CA3 region belonged largely to inhibitory cells terminating in the distal dendritic region of granule cells, as indicated by the somatostatin and neuropeptide Y (NPY) content. In addition, SPR and NPY were colocalized in numerous multipolar interneurons with dendrites branching close to the soma. Twenty-five percent of the SPR-immunoreactive cells overlapped with calretinin-positive neurons in all hippocampal subfields, showing that interneurons specialized to contact other gamma-aminobutyric acid-ergic cells may also contain SPR. On the basis of the known termination pattern of the colocalized markers, we conclude that SPR-positive interneurons are functionally heterogeneous and participate in different inhibitory processes: (1) perisomatic inhibition of principal cells (CCK-containing cells, and parvalbumin-positive cells in the dentate gyrus), (2) feedback dendritic inhibition in the entorhinal termination zone (somatostatin and NPY-containing cells), and (3) innervation of other interneurons (calretinin-containing cells).

Original languageEnglish
Pages (from-to)320-336
Number of pages17
JournalJournal of Comparative Neurology
Volume378
Issue number3
DOIs
Publication statusPublished - Feb 17 1997

Fingerprint

Neurokinin-1 Receptors
Hippocampus
Interneurons
Neuropeptide Y
Calbindin 2
Parvalbumins
Cholecystokinin
Dentate Gyrus
Dendrites
Somatostatin
Neurons
Hippocampal CA3 Region
Carisoprodol
Neurosciences
Cerebral Cortex
gamma-Aminobutyric Acid
Immune Sera

Keywords

  • colocalization
  • interneurons
  • neuropeptides
  • receptors
  • tachykinins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Immunostaining for substance P receptor labels GABAergic cells with distinct termination patterns in the hippocampus. / Acsády, L.; Katona, I.; Gulyás, A. I.; Shigemoto, R.; Freund, T.

In: Journal of Comparative Neurology, Vol. 378, No. 3, 17.02.1997, p. 320-336.

Research output: Contribution to journalArticle

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AU - Freund, T.

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