Immunomorphological characterization and effects of 12-(S)-HETE on a dynamic intracellular pool of the αIIbβ3-integrin in melanoma cells

J. Tímár, R. Bazaz, V. Kimler, M. Haddad, D. G. Tang, D. Robertson, J. Tóvári, J. D. Taylor, K. V. Honn

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

In metastatic B16a murine melanoma cells, αIIbβ3 integrin was shown to be one of the key adhesion molecules responsible for matrix adhesion and spreading. Upon stimulation, αIIbβ3 can be upregulated at the cell surface due to translocation of the receptor to the plasma membrane from an intracellular pool. Here we have characterized this integrin pool as a tubulovesicular structure (TVS) corresponding to endosomes. TVS was found to be associated temporarily with microtubules and intermediate filaments especially after protein kinase C (PKC) stimulation with a lipoxygenase metabolite of arachidonic acid, 12-(S)-hydroxyeicosatetraenoic acid [12-(S)-HETE]. After PKC stimulation, the predominantly vesicular TVS became elongated and αIIbβ3 appeared at the apical plasma membrane and microvilli. Disruption of either the microtubules or intermediate filaments prevented the 12-(S)-HETE effect both on vesicular to tubular transition of TVS as well as on surface expression of this integrin. The connection with the Golgi system of the integrin-containing TVS was proved by a Golgi-inhibitor (brefeldin A) pretreatment, which prevented the PKC-stimulation-induced TVS elongation and subsequent receptor-upregulation at the cell surface. After a soluble ligand binding (mAb to the αIIbβ3 complex) the surface receptor endocytosed back to the TVS indicating the presence of a dynamic, cytoskeleton associated integrin pool in melanoma cells.

Original languageEnglish
Pages (from-to)2175-2186
Number of pages12
JournalJournal of Cell Science
Volume108
Issue number6
Publication statusPublished - Jun 1995

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12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
Integrins
Melanoma
Protein Kinase C
Intermediate Filaments
Microtubules
Cell Membrane
Brefeldin A
Lipoxygenase
Endosomes
Microvilli
Endocytosis
Cytoskeleton
Arachidonic Acid
Up-Regulation
Ligands

Keywords

  • αβ
  • 12(S)-HETE
  • Melanoma
  • Tubulovesicular structure

ASJC Scopus subject areas

  • Cell Biology

Cite this

Immunomorphological characterization and effects of 12-(S)-HETE on a dynamic intracellular pool of the αIIbβ3-integrin in melanoma cells. / Tímár, J.; Bazaz, R.; Kimler, V.; Haddad, M.; Tang, D. G.; Robertson, D.; Tóvári, J.; Taylor, J. D.; Honn, K. V.

In: Journal of Cell Science, Vol. 108, No. 6, 06.1995, p. 2175-2186.

Research output: Contribution to journalArticle

Tímár, J, Bazaz, R, Kimler, V, Haddad, M, Tang, DG, Robertson, D, Tóvári, J, Taylor, JD & Honn, KV 1995, 'Immunomorphological characterization and effects of 12-(S)-HETE on a dynamic intracellular pool of the αIIbβ3-integrin in melanoma cells', Journal of Cell Science, vol. 108, no. 6, pp. 2175-2186.
Tímár, J. ; Bazaz, R. ; Kimler, V. ; Haddad, M. ; Tang, D. G. ; Robertson, D. ; Tóvári, J. ; Taylor, J. D. ; Honn, K. V. / Immunomorphological characterization and effects of 12-(S)-HETE on a dynamic intracellular pool of the αIIbβ3-integrin in melanoma cells. In: Journal of Cell Science. 1995 ; Vol. 108, No. 6. pp. 2175-2186.
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