Immunomodulatory potential of differently-terminated ultra-small silicon carbide nanoparticles

Tereza Bĕlinová, Iva Machová, David Beke, Anna Fučíková, Adam Gali, Zuzana Humlová, Jan Valenta, Marie Hubálek Kalbáčová

Research output: Contribution to journalArticle

Abstract

Ultra-small nanoparticles with sizes comparable to those of pores in the cellular membrane possess significant potential for application in the field of biomedicine. Silicon carbide ultra-small nanoparticles with varying surface termination were tested for the biological system represented by different human cells (using a human osteoblastic cell line as the reference system and a monocyte/macrophage cell line as immune cells). The three tested nanoparticle surface terminations resulted in the observation of different effects on cell metabolic activity. These effects were mostly noticeable in cases of monocytic cells, where each type of particle caused a completely different response (‘as-prepared’ particles, i.e., were highly cytotoxic, -OH terminated particles slightly increased the metabolic activity, while -NH2 terminated particles caused an almost doubled metabolic activity) after 24 h of incubation. Subsequently, the release of cytokines from such treated monocytes and their differentiation into activated cells was determined. The results revealed the potential modulation of immune cell behavior following stimulation with particular ultra-small nanoparticles, thus opening up new fields for novel silicon carbide nanoparticle biomedical applications.

Original languageEnglish
Article number573
JournalNanomaterials
Volume10
Issue number3
DOIs
Publication statusPublished - Mar 2020

Keywords

  • Cytotoxicity
  • Immune cells
  • Nanoparticles
  • Osteoblasts
  • Silicon carbide

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Materials Science(all)

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    Bĕlinová, T., Machová, I., Beke, D., Fučíková, A., Gali, A., Humlová, Z., Valenta, J., & Kalbáčová, M. H. (2020). Immunomodulatory potential of differently-terminated ultra-small silicon carbide nanoparticles. Nanomaterials, 10(3), [573]. https://doi.org/10.3390/nano10030573