Immunomodulatory effect of synthetic peptides corresponding to sequences within the CH2 and CH3 domain of human IgG1

G. Sármay, Krisztina Reguly, Robert Szigeti, Eva Klein, Denis R. Stanworth, J. Gergely

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The Fc region of IgG is known to be the source of small peptides possessing immunomodulatory function. Results are summarized showing the effect of synthetic peptides composed of surface exposed residues of Cγ 2 or Cγ 3 domains on different steps of human B lymphocyte activation cycle. Both the CH2 (289Thr-301Arg) and CH3 (407Tyr-416Arg) peptides as well as the whole Fc fragment enhanced the IgM synthesis of PWM or PMA + CaI activated lymphocytes. This effect was exerted at the early phase of B cell activation. The incubation of separated resting B cells with Fc fragments or CH2 peptides resulted in increase of cell volume and in expression of HLA-DR antigen. On the other hand, OF production was induced both by CH2 and CH3 peptides. It was also shown that Fc peptides induce IL-1 release from monocytes. The results suggest that the CH2 and CH3 domain peptides exert their effect partly directly, by activating resting B cells, rendering the cells more susceptible to other stimuli; and moreover, by enhancing the humoral response by triggering the release of IL-1.

Original languageEnglish
Pages (from-to)1183-1188
Number of pages6
JournalMolecular Immunology
Volume25
Issue number11
DOIs
Publication statusPublished - 1988

Fingerprint

Immunoglobulin G
Peptides
B-Lymphocytes
Immunoglobulin Fc Fragments
Interleukin-1
HLA-DR Antigens
Lymphocyte Activation
Cell Size
Immunoglobulin M
Monocytes
Lymphocytes

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

Cite this

Immunomodulatory effect of synthetic peptides corresponding to sequences within the CH2 and CH3 domain of human IgG1. / Sármay, G.; Reguly, Krisztina; Szigeti, Robert; Klein, Eva; Stanworth, Denis R.; Gergely, J.

In: Molecular Immunology, Vol. 25, No. 11, 1988, p. 1183-1188.

Research output: Contribution to journalArticle

Sármay, G. ; Reguly, Krisztina ; Szigeti, Robert ; Klein, Eva ; Stanworth, Denis R. ; Gergely, J. / Immunomodulatory effect of synthetic peptides corresponding to sequences within the CH2 and CH3 domain of human IgG1. In: Molecular Immunology. 1988 ; Vol. 25, No. 11. pp. 1183-1188.
@article{ad47fede8995492c912da606bc611b27,
title = "Immunomodulatory effect of synthetic peptides corresponding to sequences within the CH2 and CH3 domain of human IgG1",
abstract = "The Fc region of IgG is known to be the source of small peptides possessing immunomodulatory function. Results are summarized showing the effect of synthetic peptides composed of surface exposed residues of Cγ 2 or Cγ 3 domains on different steps of human B lymphocyte activation cycle. Both the CH2 (289Thr-301Arg) and CH3 (407Tyr-416Arg) peptides as well as the whole Fc fragment enhanced the IgM synthesis of PWM or PMA + CaI activated lymphocytes. This effect was exerted at the early phase of B cell activation. The incubation of separated resting B cells with Fc fragments or CH2 peptides resulted in increase of cell volume and in expression of HLA-DR antigen. On the other hand, OF production was induced both by CH2 and CH3 peptides. It was also shown that Fc peptides induce IL-1 release from monocytes. The results suggest that the CH2 and CH3 domain peptides exert their effect partly directly, by activating resting B cells, rendering the cells more susceptible to other stimuli; and moreover, by enhancing the humoral response by triggering the release of IL-1.",
author = "G. S{\'a}rmay and Krisztina Reguly and Robert Szigeti and Eva Klein and Stanworth, {Denis R.} and J. Gergely",
year = "1988",
doi = "10.1016/0161-5890(88)90154-X",
language = "English",
volume = "25",
pages = "1183--1188",
journal = "Molecular Immunology",
issn = "0161-5890",
publisher = "Elsevier Limited",
number = "11",

}

TY - JOUR

T1 - Immunomodulatory effect of synthetic peptides corresponding to sequences within the CH2 and CH3 domain of human IgG1

AU - Sármay, G.

AU - Reguly, Krisztina

AU - Szigeti, Robert

AU - Klein, Eva

AU - Stanworth, Denis R.

AU - Gergely, J.

PY - 1988

Y1 - 1988

N2 - The Fc region of IgG is known to be the source of small peptides possessing immunomodulatory function. Results are summarized showing the effect of synthetic peptides composed of surface exposed residues of Cγ 2 or Cγ 3 domains on different steps of human B lymphocyte activation cycle. Both the CH2 (289Thr-301Arg) and CH3 (407Tyr-416Arg) peptides as well as the whole Fc fragment enhanced the IgM synthesis of PWM or PMA + CaI activated lymphocytes. This effect was exerted at the early phase of B cell activation. The incubation of separated resting B cells with Fc fragments or CH2 peptides resulted in increase of cell volume and in expression of HLA-DR antigen. On the other hand, OF production was induced both by CH2 and CH3 peptides. It was also shown that Fc peptides induce IL-1 release from monocytes. The results suggest that the CH2 and CH3 domain peptides exert their effect partly directly, by activating resting B cells, rendering the cells more susceptible to other stimuli; and moreover, by enhancing the humoral response by triggering the release of IL-1.

AB - The Fc region of IgG is known to be the source of small peptides possessing immunomodulatory function. Results are summarized showing the effect of synthetic peptides composed of surface exposed residues of Cγ 2 or Cγ 3 domains on different steps of human B lymphocyte activation cycle. Both the CH2 (289Thr-301Arg) and CH3 (407Tyr-416Arg) peptides as well as the whole Fc fragment enhanced the IgM synthesis of PWM or PMA + CaI activated lymphocytes. This effect was exerted at the early phase of B cell activation. The incubation of separated resting B cells with Fc fragments or CH2 peptides resulted in increase of cell volume and in expression of HLA-DR antigen. On the other hand, OF production was induced both by CH2 and CH3 peptides. It was also shown that Fc peptides induce IL-1 release from monocytes. The results suggest that the CH2 and CH3 domain peptides exert their effect partly directly, by activating resting B cells, rendering the cells more susceptible to other stimuli; and moreover, by enhancing the humoral response by triggering the release of IL-1.

UR - http://www.scopus.com/inward/record.url?scp=0023779407&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023779407&partnerID=8YFLogxK

U2 - 10.1016/0161-5890(88)90154-X

DO - 10.1016/0161-5890(88)90154-X

M3 - Article

VL - 25

SP - 1183

EP - 1188

JO - Molecular Immunology

JF - Molecular Immunology

SN - 0161-5890

IS - 11

ER -