Immunomodulatory capacity of the serotonin receptor 5-HT2B in a subset of human dendritic cells

Attila Szabo, P. Gogolák, G. Koncz, Zsofia Foldvari, Kitti Pazmandi, Noemi Miltner, Szilard Poliska, Attila Bacsi, Srdjan Djurovic, E. Rajnavolgyi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Serotonin is a monoamine neurotransmitter that signals through a wide array of receptors (5-HT1-7) many of which are also involved in immune processes. Dendritic cells (DCs) are crucial players in immune defense by bridging innate and adaptive immune responses via their vast repertoire of pattern recognition receptors and antigen-presenting capability. Although serotonin is known to influence immunity at many levels, cell type-specific expression and function of its receptors remains poorly understood. Here we aimed to study 5-HT1-7 expression and function in CD1a- and CD1a+ human monocyte-derived DCs (moDCs). We found that the 5-HT2B receptor-subtype is solely expressed by the inflammatory CD1a+ moDC subset. Specific 5-HT2B activation potently inhibited TLR2, TLR3, and TLR7/8-induced proinflammatory cytokine and chemokine (TNF-α, IL-6, IL-8, IP-10, IL-12) but not type I interferon-β responses. 5-HT2B agonism also interfered with the polarization of CD1a+ moDC-primed CD4+ T cells towards inflammatory Th1 and Th17 effector lymphocytes. Here we report the subsetspecific expression and immunomodulatory function of 5-HT2B in human moDCs. Our results expand the biological role of 5-HT2B which may act not only as a neurotransmitter receptor, but also as an important modulator of both innate and adaptive immune responses.

Original languageEnglish
Article number1765
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - Dec 1 2018

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Receptor, Serotonin, 5-HT2B
Adaptive Immunity
Innate Immunity
Dendritic Cells
Monocytes
Serotonin
Serotonin 5-HT1 Receptors
Pattern Recognition Receptors
Interferon Type I
Neurotransmitter Receptor
Interleukin-12
Interleukin-8
Chemokines
Neurotransmitter Agents
Immunity
Interleukin-6
Lymphocytes
Cytokines
T-Lymphocytes
Antigens

ASJC Scopus subject areas

  • General

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Immunomodulatory capacity of the serotonin receptor 5-HT2B in a subset of human dendritic cells. / Szabo, Attila; Gogolák, P.; Koncz, G.; Foldvari, Zsofia; Pazmandi, Kitti; Miltner, Noemi; Poliska, Szilard; Bacsi, Attila; Djurovic, Srdjan; Rajnavolgyi, E.

In: Scientific Reports, Vol. 8, No. 1, 1765, 01.12.2018.

Research output: Contribution to journalArticle

Szabo, Attila ; Gogolák, P. ; Koncz, G. ; Foldvari, Zsofia ; Pazmandi, Kitti ; Miltner, Noemi ; Poliska, Szilard ; Bacsi, Attila ; Djurovic, Srdjan ; Rajnavolgyi, E. / Immunomodulatory capacity of the serotonin receptor 5-HT2B in a subset of human dendritic cells. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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