Immunological and biochemical characterisation of 7ap, a short protein translated from an alternative frame of ORF7 of PRRSV

F. Olasz, B. Dénes, A. Bálint, Tibor Magyar, Sándor Belák, Z. Zádori

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Sequence analysis revealed a short alternative open reading frame (ORF) named ORF7a within the nucleocapsid gene of genetically divergent porcine reproductive and respiratory syndrome virus (PRRSV) genomes. Alignment of the corresponding protein sequences (named 7ap) revealed substantial heterogeneity among 7aps of different genotypes, though all of them are predicted to be positively charged. Green fluorescent protein and FLAG fusion constructs of ORF7a of the HU-14432/2011 PRRSV demonstrated that 7ap is expressed. 7ap of HU-14432/2011 (Hu7ap) was synthesised chemically, and ELISA experiments revealed that Hu7ap binds strongly to mammalian IgGs. Protein-protein gel retardation assays and complement fixation inhibition suggest that 7aps bind to the CH2 domain of the IgG(Fc) fragment. Cellular localisation and immunological characteristics of PRRSV 7ap may indicate multiple functions including nuclear and cytoplasmic over-tuning of normal cellular processes and immunosuppression.

Original languageEnglish
Pages (from-to)273-287
Number of pages15
JournalActa Veterinaria Hungarica
Volume64
Issue number2
DOIs
Publication statusPublished - Jun 1 2016

Fingerprint

Porcine respiratory and reproductive syndrome virus
Porcine reproductive and respiratory syndrome virus
Immunoglobulin Fc Fragments
Nucleocapsid
nucleocapsid
Proteins
proteins
immunosuppression
Electrophoretic Mobility Shift Assay
Green Fluorescent Proteins
green fluorescent protein
Immunosuppression
Open Reading Frames
Sequence Analysis
open reading frames
complement
amino acid sequences
sequence analysis
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay

Keywords

  • Complement fixation inhibition
  • IgG binding
  • Overlapping ORF
  • Positive charged short peptide
  • PRRSV

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Immunological and biochemical characterisation of 7ap, a short protein translated from an alternative frame of ORF7 of PRRSV. / Olasz, F.; Dénes, B.; Bálint, A.; Magyar, Tibor; Belák, Sándor; Zádori, Z.

In: Acta Veterinaria Hungarica, Vol. 64, No. 2, 01.06.2016, p. 273-287.

Research output: Contribution to journalArticle

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AU - Belák, Sándor

AU - Zádori, Z.

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