Immunological alterations in newly diagnosed primary Sjogren's syndrome characterized by skewed peripheral T-cell subsets and inflammatory cytokines

P. Szodoray, I. Gal, S. Baráth, M. Aleksza, I. F. Horvath, P. Gergely, G. Szegedi, B. Nakken, M. Zeher

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Objectives: To describe how certain peripheral immune parameters reflect the inflammatory alterations in patients with primary Sjogren's syndrome (pSS). We determined lymphocyte subpopulations and their state of activation from peripheral blood, evaluating both soluble serum T-helper (Th)1/Th2-type cytokines and intracytoplasmic cytokines. Methods: Forty-nine patients with newly diagnosed pSS and 40 healthy individuals, all free from immunomodulant or immunosuppressive medication, were studied. Peripheral blood lymphocyte subgroups were quantified by flow cytometry, soluble cytokines were assessed using enzyme-linked immunosorbent assay (ELISA), and intracellular cytokine levels were measured after phorbol myristate acetate (PMA) stimulation by flow cytometry after staining of intracellular cytokines. Results: Patients with primary SS had higher percentages of activated CD3+/CD69+ T cells than controls. When comparing naive vs. memory subsets of CD4+ and CD8+ T cells, a shift towards the memory phenotype was observed for both. Natural killer (NK) cell and NK T-cell (NKT) percentages and Th0 and Th1 cell numbers were increased in patients compared to controls. Among circulating cytokines, interferon (IFN)-γ was high, whereas interleukin (IL)-10 was decreased in SS when compared to controls. Conclusions: SS, considered as a systemic autoimmune disease, is characterized by a complex interplay of various cytokines and immune cells. The skewed T-cell subsets and cytokine imbalance play important roles in an orchestrated proinflammatory cascade.

Original languageEnglish
Pages (from-to)205-212
Number of pages8
JournalScandinavian Journal of Rheumatology
Volume37
Issue number3
DOIs
Publication statusPublished - 2008

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Sjogren's Syndrome
T-Lymphocyte Subsets
Cytokines
Flow Cytometry
T-Lymphocytes
Th1 Cells
Natural Killer T-Cells
Lymphocyte Subsets
Tetradecanoylphorbol Acetate
Immunosuppressive Agents
Natural Killer Cells
Interleukin-10
Interferons
Autoimmune Diseases
Cell Count
Enzyme-Linked Immunosorbent Assay
Lymphocytes
Staining and Labeling
Phenotype
Serum

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Cite this

Immunological alterations in newly diagnosed primary Sjogren's syndrome characterized by skewed peripheral T-cell subsets and inflammatory cytokines. / Szodoray, P.; Gal, I.; Baráth, S.; Aleksza, M.; Horvath, I. F.; Gergely, P.; Szegedi, G.; Nakken, B.; Zeher, M.

In: Scandinavian Journal of Rheumatology, Vol. 37, No. 3, 2008, p. 205-212.

Research output: Contribution to journalArticle

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AU - Gal, I.

AU - Baráth, S.

AU - Aleksza, M.

AU - Horvath, I. F.

AU - Gergely, P.

AU - Szegedi, G.

AU - Nakken, B.

AU - Zeher, M.

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