Immunohistochemistry of cerebellar seizures

mossy fiber afferents play an important role in seizure spread and initiation in the rat

Zoltán Tóth, Gergely Molnár, A. Mihály, B. Krisztin-Péva, Marietta Morvai, Zsolt Kopniczky

Research output: Contribution to journalArticle

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Abstract

Clinical reports suggest the participation of the cerebellum in epilepsy. Mossy fibers are the main excitatory afferents of the cerebellar cortex; most of them use glutamate and strongly excite granule cells through NMDA and AMPA receptors. The role of the ponto-cerebellar mossy fibers in cerebellar neuronal hyperactivity was investigated in the present study in experimental adult Wistar rats. We detected neuronal hyperactivity through the expression of the glutamate-induced c-fos protein, by means of immunohistochemistry and immunoblotting in the vermis and in the hemispheres. Generalized seizures were induced by means of intraperitoneal 4-aminopyridine injections. Following the 4-aminopyridine seizures, the c-fos expression of cerebellar granule cells was significantly elevated at 1.5h in every lobule. Maximum c-fos expression was seen at 3h. The role of the ponto-cerebellar mossy fiber afferents in the induction of c-fos expression was examined after the transection of the middle cerebellar peduncle on the left side. Immunohistochemical analysis 14 days after the surgery revealed that the synapsin I immunoreactivity was significantly reduced in the cerebellar cortex on the operated side, compared to the sham-operated controls and to the non-operated cerebellar hemisphere of the operated animals, indicating the degeneration of mossy fiber terminals. Transection of the middle cerebellar peduncle suppressed cerebellar c-fos expression in the vermis and in the hemispheres significantly. These findings suggest the strong involvement of the middle cerebellar peduncle and the ponto-cerebellar mossy fibers in the pathophysiology of cerebellar epilepsy.

Original languageEnglish
Pages (from-to)47-55
Number of pages9
JournalActa Histochemica
Volume117
Issue number1
DOIs
Publication statusPublished - Jan 1 2015

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Nerve Fibers
4-Aminopyridine
Cerebellar Cortex
Seizures
Immunohistochemistry
Glutamic Acid
Epilepsy
Synapsins
Proto-Oncogene Proteins c-fos
AMPA Receptors
N-Methyl-D-Aspartate Receptors
Ambulatory Surgical Procedures
Immunoblotting
Cerebellum
Wistar Rats
Injections
Middle Cerebellar Peduncle

Keywords

  • c-fos
  • Cerebellar cortex
  • Epilepsy
  • Immunohistochemistry
  • Middle cerebellar peduncle
  • Rat.

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Immunohistochemistry of cerebellar seizures : mossy fiber afferents play an important role in seizure spread and initiation in the rat. / Tóth, Zoltán; Molnár, Gergely; Mihály, A.; Krisztin-Péva, B.; Morvai, Marietta; Kopniczky, Zsolt.

In: Acta Histochemica, Vol. 117, No. 1, 01.01.2015, p. 47-55.

Research output: Contribution to journalArticle

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