The authors present a descriptive immunohistochemical study of different intact tissues and organs in ferrets. In the study 20 tissue samples were taken from lesion-free organs of 5 ferrets and were examined by immunohistochemical methods using a mesenchymal marker, vimentin. The aim of the study was to examine which organs or tissues show reliable vimentin positivity. In the course of necropsy examination samples were collected from the tissues of the heart, lymph node, spleen, thymus, oesophagus, stomach, duodenum, colon, liver, pancreas, trachea, lung, kidney, urinary bladder, hypophysis, adrenal gland, brain, peripheral nerve, skin and diaphragm. The samples were fixed in 8% neutral buffered formaldehyde solution and after 24 hours they were embedded in paraffin wax. From each paraffin block 4 μn thick sections were cut and the sections were immunostained using the EnVision System with a monoclonal mouse antibody, antivimentin. The result of the study revealed that the endothelial cells of blood and lymphvessels, the myoepithelial cells of acini, the fat cells, the lymphoid cells of all the examined organs showed strong positive reaction for vimentin. The adrenal cortex cells, the glial cells of the brain, the meningothelial- and smooth muscle cells of the leptomeninges demonstrated homogenous cytoplasmatic vimentin positive reaction. Surprisingly the cardiac muscle and the striated muscle were negative for vimentin. The nerve cells, the epithelial tissue, the cells of medullar region of the adrenal gland, the cells of liver, the exocrine part and Langerhans islands of the pancreas were negative for vimentin.
|Number of pages||7|
|Journal||Magyar Allatorvosok Lapja|
|Publication status||Published - Jul 1 2010|
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