Immunohistochemical localization of the neuropeptide, pituitary adenylate cyclase activating polypeptide (PACAP), in human and primate hypothalamus

Sándor Vigh, Akira Arimura, K. Köves, Anikó Somogyvári-Vigh, John Sitton, Cesar D. Fermin

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

A 38 residue neuropeptide was recently isolated from ovine hypothalamus in our laboratory, and named pituitary adenylate cyclase activating polypeptide (PACAP38) based on its biological activity. Rabbit antisera against synthetic PACAP27 were characterized by ELISA for immunohistochemical use. PACAP-immunoreactive neuronal elements having similar distributions were demonstrated in both human and spider monkey hypothalami. Many PACAP-immunoreactive cell bodies were present in the supraoptic and paraventricular nuclei. Immunopositive nerve fiber networks were stained throughout the hypothalamus, including in both external and internal zones of the tuber cinereum, close to the transition of the pituitary stalk (median eminence). These results suggest that PACAP plays multifunctional roles as a hypophysiotropic hormone, neurohypophysial hormone, neurotransmitter or neuromodulator in higher vertebrate species including man.

Original languageEnglish
Pages (from-to)313-318
Number of pages6
JournalPeptides
Volume12
Issue number2
DOIs
Publication statusPublished - 1991

Fingerprint

Pituitary Adenylate Cyclase-Activating Polypeptide
Neuropeptides
Primates
Hypothalamus
Neurotransmitter Agents
Tuber Cinereum
Atelinae
Posterior Pituitary Hormones
Pituitary Hormone-Releasing Hormones
Supraoptic Nucleus
Median Eminence
Paraventricular Hypothalamic Nucleus
Pituitary Gland
Bioactivity
Nerve Fibers
Vertebrates
Immune Sera
Sheep
Enzyme-Linked Immunosorbent Assay
Cells

Keywords

  • Human and primate hypothalamus
  • Immunohistochemistry
  • PACAP

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Immunohistochemical localization of the neuropeptide, pituitary adenylate cyclase activating polypeptide (PACAP), in human and primate hypothalamus. / Vigh, Sándor; Arimura, Akira; Köves, K.; Somogyvári-Vigh, Anikó; Sitton, John; Fermin, Cesar D.

In: Peptides, Vol. 12, No. 2, 1991, p. 313-318.

Research output: Contribution to journalArticle

Vigh, Sándor ; Arimura, Akira ; Köves, K. ; Somogyvári-Vigh, Anikó ; Sitton, John ; Fermin, Cesar D. / Immunohistochemical localization of the neuropeptide, pituitary adenylate cyclase activating polypeptide (PACAP), in human and primate hypothalamus. In: Peptides. 1991 ; Vol. 12, No. 2. pp. 313-318.
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