Immunohistochemical localization of somatostatin receptor SST2A in the rat pancreas

B. Hunyady, R. William Hipkin, Agnes Schonbrunn, Éva Mezey

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Somatostatin (SRIF) acts on specific membrane receptors to inhibit exocrine and endocrine pancreatic functions. Five SRIF receptor genes have been cloned, producing six receptor proteins (sst-s). We used a recently developed antibody to localize the sst2A splice variant in the rat pancreas. Western blots identified the sst2A receptor as an 90 kDa glycosylated protein in pancreatic tissue. In tyramide-amplified immunostainings all acinar cells, and the glucagon and pancreatic polypeptide immunoreactive cells (A and PP, respectively) were intensely labeled for sst2A, while no signal was detected in SRIF producing (D) cells. A very few insulin immunoreactive (B) cells were also labeled for sst2A, but the signal in these cells was lower than in exocrine, A or PP cells. Absorption of the sst2A antibody with the receptor peptide abolished specific staining in both immunoblots and tissue sections (negative control). These studies are the first to localize any SRIF receptor subtype, in the rat pancreas. The specific localization of sst2A receptor of acinar. A and PP cell if confirmed ed in humans, would suggest that subtype specific analogs will be useful for the therapeutic regulation of exocrine and/or endocrine pancreatic secretion.

Original languageEnglish
Pages (from-to)2632-2635
Number of pages4
JournalEndocrinology
Volume138
Issue number6
DOIs
Publication statusPublished - 1997

Fingerprint

Somatostatin Receptors
Pancreas
Pancreatic Polypeptide-Secreting Cells
Peptide Receptors
Somatostatin-Secreting Cells
Antibodies
Acinar Cells
Somatostatin
Glucagon
Proteins
B-Lymphocytes
Western Blotting
Insulin
Staining and Labeling
Membranes
Genes
somatostatin receptor sst2A
Therapeutics

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Immunohistochemical localization of somatostatin receptor SST2A in the rat pancreas. / Hunyady, B.; Hipkin, R. William; Schonbrunn, Agnes; Mezey, Éva.

In: Endocrinology, Vol. 138, No. 6, 1997, p. 2632-2635.

Research output: Contribution to journalArticle

Hunyady, B. ; Hipkin, R. William ; Schonbrunn, Agnes ; Mezey, Éva. / Immunohistochemical localization of somatostatin receptor SST2A in the rat pancreas. In: Endocrinology. 1997 ; Vol. 138, No. 6. pp. 2632-2635.
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