Immunohistochemical localization of raf protein kinase in dendritic spines and spine apparatuses of the rat cerebral cortex

A. Mihály, Tamás Oravecz, Zoltán Oláh, Ulf R. Rapp

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The ultrastructural localization of raf protein (product of the raf protooncogene) in the neocortex, pyriform cortex and hippocampus of the rat has been investigated by means of pre-embedding immunohistochemistry. Specificity of the antiserum was tested with Western blotting. Besides the immunoreactivity of the dendrites, remarkably strong immunostaining of the dendritic spines and spine apparatuses was noted in each of the investigated areas. The postsynaptic densities were also stained. Since raf proteins are serine/threonine-specific protein kinases, our findings could be important steps toward the understanding of dendritic spine plasticity.

Original languageEnglish
Pages (from-to)309-314
Number of pages6
JournalBrain Research
Volume547
Issue number2
DOIs
Publication statusPublished - May 3 1991

Fingerprint

raf Kinases
Dendritic Spines
Cerebral Cortex
Protein Kinases
Spine
Post-Synaptic Density
Protein-Serine-Threonine Kinases
Neocortex
Dendrites
Immune Sera
Hippocampus
Proteins
Western Blotting
Immunohistochemistry

Keywords

  • Dendritic spine
  • Immunohistochemistry
  • Protein kinase
  • Protooncogene
  • Spine apparatus

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Immunohistochemical localization of raf protein kinase in dendritic spines and spine apparatuses of the rat cerebral cortex. / Mihály, A.; Oravecz, Tamás; Oláh, Zoltán; Rapp, Ulf R.

In: Brain Research, Vol. 547, No. 2, 03.05.1991, p. 309-314.

Research output: Contribution to journalArticle

@article{0371bc7d73aa4a5681c1a710e17772ed,
title = "Immunohistochemical localization of raf protein kinase in dendritic spines and spine apparatuses of the rat cerebral cortex",
abstract = "The ultrastructural localization of raf protein (product of the raf protooncogene) in the neocortex, pyriform cortex and hippocampus of the rat has been investigated by means of pre-embedding immunohistochemistry. Specificity of the antiserum was tested with Western blotting. Besides the immunoreactivity of the dendrites, remarkably strong immunostaining of the dendritic spines and spine apparatuses was noted in each of the investigated areas. The postsynaptic densities were also stained. Since raf proteins are serine/threonine-specific protein kinases, our findings could be important steps toward the understanding of dendritic spine plasticity.",
keywords = "Dendritic spine, Immunohistochemistry, Protein kinase, Protooncogene, Spine apparatus",
author = "A. Mih{\'a}ly and Tam{\'a}s Oravecz and Zolt{\'a}n Ol{\'a}h and Rapp, {Ulf R.}",
year = "1991",
month = "5",
day = "3",
doi = "10.1016/0006-8993(91)90976-3",
language = "English",
volume = "547",
pages = "309--314",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Immunohistochemical localization of raf protein kinase in dendritic spines and spine apparatuses of the rat cerebral cortex

AU - Mihály, A.

AU - Oravecz, Tamás

AU - Oláh, Zoltán

AU - Rapp, Ulf R.

PY - 1991/5/3

Y1 - 1991/5/3

N2 - The ultrastructural localization of raf protein (product of the raf protooncogene) in the neocortex, pyriform cortex and hippocampus of the rat has been investigated by means of pre-embedding immunohistochemistry. Specificity of the antiserum was tested with Western blotting. Besides the immunoreactivity of the dendrites, remarkably strong immunostaining of the dendritic spines and spine apparatuses was noted in each of the investigated areas. The postsynaptic densities were also stained. Since raf proteins are serine/threonine-specific protein kinases, our findings could be important steps toward the understanding of dendritic spine plasticity.

AB - The ultrastructural localization of raf protein (product of the raf protooncogene) in the neocortex, pyriform cortex and hippocampus of the rat has been investigated by means of pre-embedding immunohistochemistry. Specificity of the antiserum was tested with Western blotting. Besides the immunoreactivity of the dendrites, remarkably strong immunostaining of the dendritic spines and spine apparatuses was noted in each of the investigated areas. The postsynaptic densities were also stained. Since raf proteins are serine/threonine-specific protein kinases, our findings could be important steps toward the understanding of dendritic spine plasticity.

KW - Dendritic spine

KW - Immunohistochemistry

KW - Protein kinase

KW - Protooncogene

KW - Spine apparatus

UR - http://www.scopus.com/inward/record.url?scp=0025777486&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025777486&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(91)90976-3

DO - 10.1016/0006-8993(91)90976-3

M3 - Article

VL - 547

SP - 309

EP - 314

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 2

ER -