Immunohistochemical identification of tubular segments in percutaneous renal biopsies

B. Iványi, T. Steen Olsen

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

To identify the renal cortical tubular segments involved in tubulo-interstitial disease in formalin-fixed, paraffin-embedded percutaneous kidney biopsies, we developed multiple immunolabeling protocols using segment-specific tubular markers. The present study of biopsies from patients with minimal change or thin basement membrane nephropathy provides a baseline for interpretation of histopathology. Proximal tubules were stained either by the PAS reaction or by the biotinylated Phaseolus vulgaris erythroagglutinin (PHA-E)-streptavidin-gold-silver system (brush borders black). The anti-Tamm-Horsfall (THP) antibody-immunoperoxidase (aminoethylcarbazole, AEC-IPO), and anti-epidermal cytokeratins (ECK) antibodies-immunoalkaline-Fast Blue BB methods marked the distal straight tubules and the cortical collecting system red-brown and blue, respectively. When these immunolabelings were combined, the coapplication of AEC-PO-labeled peanut agglutinin (PNA) or anti-epithelial membrane antigen antibody-AEC-IPO technique (both are markers for distal nephron) visualized the apical membranes of distal convoluted tubules. In the protocol PHA-E + PNA + THP + ECK, the tubular basement membranes were outlined by the anti-laminin antibody-AEC-IPO staining, carried out simultaneously. The protocol PNA + THP + ECK + PAS was found to be a quite appropriate multiple immunolabeling method for the tubules, and is recommended for use as a tool in the study of tubulo-interstitial diseases.

Original languageEnglish
Pages (from-to)351-356
Number of pages6
JournalHistochemistry
Volume95
Issue number4
DOIs
Publication statusPublished - Feb 1991

Fingerprint

Peanut Agglutinin
Keratins
Phaseolus
Kidney
Biopsy
Antibodies
Periodic Acid-Schiff Reaction
Mucin-1
Streptavidin
Nephrons
Laminin
Microvilli
Silver
Basement Membrane
Gold
Paraffin
Formaldehyde
Anti-Idiotypic Antibodies
Staining and Labeling
Membranes

ASJC Scopus subject areas

  • Anatomy

Cite this

Immunohistochemical identification of tubular segments in percutaneous renal biopsies. / Iványi, B.; Olsen, T. Steen.

In: Histochemistry, Vol. 95, No. 4, 02.1991, p. 351-356.

Research output: Contribution to journalArticle

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