Immunohistochemical detection of raf protein kinase in cerebral cortical areas of adult guinea pigs and rats

A. Mihály, V. Endrész, Tamás Oravecz, Ulf R. Rapp, Ulrich Kuhnt

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The raf protooncogenes are the cellular counterparts of the v-raf oncogene expressed by a murine sarcoma virus. The raf protooncogenes encode cytoplasmic serine/threonine-specific protein kinases which can be activated from different factor receptors by phosphorylation The mRNAs of raf protooncogenes are found in a large variety of normal adult tissues, including the central nervous system. As concerns the distribution and localization of their protein products (the raf kinases), very few data are to be found in the literature. This is the first detailed description of their light microscopic localization in neocortical and allocortical areas of rodents. Preembedding immunohistochemical studies were performed on vibratome sections from the brains of adult guinea pigs and albino rats. The localizations of two isoenzymes, raf-1 kinase and B-raf kinase, were studied with the help of isoenzyme-specific polyclonal antibodies. Both of the antibodies detected raf protein-like immunoreactivity in many neurons and scattered glial cells of the sensory neocortex, and the cingular, pyriform, perirhinal and entorhinal allocortical areas. Pyramidal and non-pyramidal cells of Ammon's horn, granule cells of the dentate fascia and the large neurons in the hilar region were immunoreactive, too. The findings indicated that B-raf protein kinase and raf-1 kinase are present almost ubiquitously in the neurons of the investigated cortical structures. The intensity of staining obtained with serial dilutions of the antibodies indicated that the cytoplasmic concentration of B-raf kinase is tended to be higher than that of raf-1 kinase. The present findings suggested that the raf kinases are localized in postsynaptic structures, mainly in dendrites and cell bodies. Their cytosolic localization and their ability to undergo intracellular translocation during activation and phosphorylation raise the possibility that they play a pivotal role in the intracellular signalling of neurons.

Original languageEnglish
Pages (from-to)225-238
Number of pages14
JournalBrain Research
Volume627
Issue number2
DOIs
Publication statusPublished - Nov 12 1993

Fingerprint

raf Kinases
Proto-Oncogene Proteins c-raf
Proto-Oncogene Proteins B-raf
Protein Kinases
Guinea Pigs
Neurons
Isoenzymes
Antibodies
Murine Sarcoma Viruses
Phosphorylation
Entorhinal Cortex
Protein-Serine-Threonine Kinases
Neocortex
Dentate Gyrus
Dendrites
Oncogenes
Neuroglia
Rodentia
Hippocampus
Proteins

Keywords

  • Guinea pig
  • Hippocampus
  • Immunohistochemistry
  • Neocortex
  • Protooncogene
  • Rat

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Immunohistochemical detection of raf protein kinase in cerebral cortical areas of adult guinea pigs and rats. / Mihály, A.; Endrész, V.; Oravecz, Tamás; Rapp, Ulf R.; Kuhnt, Ulrich.

In: Brain Research, Vol. 627, No. 2, 12.11.1993, p. 225-238.

Research output: Contribution to journalArticle

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