Immunohistochemical detection of factor XIII subunit a in histiocytes of human uterus

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18 Citations (Scopus)

Abstract

As spontaneous abortion is a frequent finding in females with Factor XIII (FXIII) deficiency it has been presumed that this clotting factor is essential to normal pregnancy. FXIII subunit a (FXIII A) has been demonstrated in the homogenate of human uterus, but no information on its cellular distribution has been published, so far. In the present study first FXIII A was detected in paraformaldehyde-fixed, paraffin-embedded sections of human uterus by immunoperoxidase technique. Cells containing FXIII A were localized between collagen fibrils stained by Picrosirius Red F3B in the connective tissue. To characterize them the immunofluorescent detection of FXIII a was combined by the visualization of different marker antigens of monocytes and macrophages recognized by Leu-M3, RFD7, anti-HLA-DR and DAKO-anti-macrophage monoclonal antibodies on frozen sections. The coexpression of FXIII A with monocyte and macrophage differentiation marker antigens clearly proves that cells containing FXIII A in the uterus are monocyte-derived tissue macrophages. The results well agree with our previous findings demonstrating FXIII A in human monocytes and different types of macrophages. On the basis of these results, the presence of FXIII A does not seem to be a specificity of the uterus but a characteristic of monocyte/macrophage cell line including tissue macrophages, in general.

Original languageEnglish
Pages (from-to)169-174
Number of pages6
JournalHistochemistry
Volume91
Issue number2
DOIs
Publication statusPublished - Mar 1989

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Factor XIII
Histiocytes
Uterus
Macrophages
Differentiation Antigens
Monocytes
Factor XIII Deficiency
Blood Coagulation Factors
Frozen Sections
HLA-DR Antigens
Spontaneous Abortion
Immunoenzyme Techniques
Paraffin
Connective Tissue
Collagen
Monoclonal Antibodies
Cell Line
Pregnancy

ASJC Scopus subject areas

  • Anatomy

Cite this

Immunohistochemical detection of factor XIII subunit a in histiocytes of human uterus. / Ádány, R.; Muszbek, L.

In: Histochemistry, Vol. 91, No. 2, 03.1989, p. 169-174.

Research output: Contribution to journalArticle

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