Immunohistochemical detection of CD1a antigen in formalin-fixed and paraffin-embedded tissue sections with monoclonal antibody 010

L. Krenács, L. Tiszalvicz, T. Krenács, L. Boumsell

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

The immunoreactivity of a CD1a monoclonal antibody (MAb), denoted 010, was investigated by means of the streptavidin-biotin-peroxidase method in formalin-fixed and paraffin-embedded tissues from 47 cases. The samples comprised reactive lymphoid proliferations of skin, tonsil, and lymph node including dermatopathic lymphadenopathy and Langerhans' cell histiocytosis, Hodgkin's and non-Hodgkin's lymphomas, and thymomas. Interdigitating and dermal dendritic cells, veiled cells, Langerhans' cells, and also cortical thymocytes and their neoplastic counterparts displayed immunostaining with MAb 010 in paraffin sections. These results are identical to previous ones reported for other CD1a MAbs in fresh or frozen specimens. The findings suggest that the binding site of 010 is a fixation-resistant epitope of CD1a antigen which has not been previously identified.

Original languageEnglish
Pages (from-to)99-104
Number of pages6
JournalJournal of Pathology
Volume171
Issue number2
DOIs
Publication statusPublished - 1993

Fingerprint

Langerhans Cells
Paraffin
Dendritic Cells
Formaldehyde
Monoclonal Antibodies
Langerhans Cell Histiocytosis
Thymoma
Streptavidin
Palatine Tonsil
Thymocytes
Biotin
Hodgkin Disease
Non-Hodgkin's Lymphoma
Peroxidase
Epitopes
Lymph Nodes
Binding Sites
Skin
CD1a antigen
Lymphadenopathy

Keywords

  • CD1a antigen
  • immunohistochemistry
  • monoclonal antibody 010

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Immunohistochemical detection of CD1a antigen in formalin-fixed and paraffin-embedded tissue sections with monoclonal antibody 010. / Krenács, L.; Tiszalvicz, L.; Krenács, T.; Boumsell, L.

In: Journal of Pathology, Vol. 171, No. 2, 1993, p. 99-104.

Research output: Contribution to journalArticle

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