Immunohistochemical detection of βIG-H3 in scarring human corneas

Lili Takács, Adrienn Csutak, Erzsébet Balâzs, A. Berta

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: βIG-H3 is a recently described extracellular matrix protein that is present in various organs. In rabbit corneas, increased βIG (the rabbit form of βIG-H3) mRNA levels were shown during corneal development and wound healing. In this study, we investigated the localization of βIG-H3 protein in scarring human corneas. Methods: Corneal buttons obtained during keratoplasty were examined. Immunohistological detection using a polyclonal antipeptide antibody against the βIG-H3 protein was performed on 24 pathological corneas (9 ulcerations, 8 alkali burns, 2 perforating injuries, 5 bullous keratopathy) and 2 normal corneas. a Results: In normal corneas, strong staining was present in the basal layer of the epithelium and in the endothelium; the stromal fibers showed faint, uniform immunoreactivity. In all scarring corneas, the epithelium was usually thickened and all of its layers were reactive with the βIG-H3 antibody. The cytoplasm of the stromal fibroblasts, as well as the stromal fibers around them also showed staining with the antibody. These changes were present in all scarring corneas, irrespective of the pathological process leading to scar formation. Conclusion: These results prove, at the protein level, the presence of increased amounts of βIG-H3 at the sites of scarring in human corneas.

Original languageEnglish
Pages (from-to)529-534
Number of pages6
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Volume237
Issue number7
DOIs
Publication statusPublished - Jul 1999

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Cornea
Cicatrix
Antibodies
Epithelium
Staining and Labeling
Rabbits
Proteins
Corneal Transplantation
Extracellular Matrix Proteins
Alkalies
Pathologic Processes
Burns
Wound Healing
Endothelium
Cytoplasm
Fibroblasts
Messenger RNA
Wounds and Injuries

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Immunohistochemical detection of βIG-H3 in scarring human corneas. / Takács, Lili; Csutak, Adrienn; Balâzs, Erzsébet; Berta, A.

In: Graefe's Archive for Clinical and Experimental Ophthalmology, Vol. 237, No. 7, 07.1999, p. 529-534.

Research output: Contribution to journalArticle

Takács, Lili ; Csutak, Adrienn ; Balâzs, Erzsébet ; Berta, A. / Immunohistochemical detection of βIG-H3 in scarring human corneas. In: Graefe's Archive for Clinical and Experimental Ophthalmology. 1999 ; Vol. 237, No. 7. pp. 529-534.
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