Immunohistochemical demonstration of the intracellular localization of pituitary adenylate cyclase activating polypeptide-like immunoreactivity in the rat testis using the stamp preparation

Hitoshi Yanaihara, Sándor Vigh, Tamás Kozicz, Aniko Somogyvári-Vigh, Akira Arimura

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Radioimmunoassay has revealed an abundance of PACAP in the rat testis. In the present study, a novel stamp preparation together with light microscopic immunohistochemistry were used to investigate in detail the intracellular localization of PACAP-like immunoreactivity (PACAP-LI) in rat germ cells. Samples were obtained by pressing the freshly cut surfaces of the testes against glass slides. PACAP-LI was clearly identified in developing acrosomes using five antisera which recognize PACAP 38, or both PACAP 27 and PACAP 38. Immunoreactivity with the antisera specific to PACAP38 was strictly localized in the developing acrosomes of the spermatids but vanished in the mature spermatids. Using an antiserum which detects PACAP 27 specifically, little staining was observed. Based on the specificities of antisera used, it was suggested that the PACAP-LI in the acrosomes represents mainly PACAP 38-LI. In addition, the present results supported the usefulness of the stamp preparation for immunohistochemical study of testicular tissues. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)83-88
Number of pages6
JournalRegulatory Peptides
Volume78
Issue number1-3
DOIs
Publication statusPublished - Nov 30 1998

Fingerprint

Pituitary Adenylate Cyclase-Activating Polypeptide
Testis
Rats
Demonstrations
Acrosome
Immune Sera
Spermatids
Germ Cells
Radioimmunoassay
Glass
Immunohistochemistry

Keywords

  • Immunohistochemistry
  • PACAP
  • Rat
  • Stamp preparation
  • Testis

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Neuroscience(all)

Cite this

Immunohistochemical demonstration of the intracellular localization of pituitary adenylate cyclase activating polypeptide-like immunoreactivity in the rat testis using the stamp preparation. / Yanaihara, Hitoshi; Vigh, Sándor; Kozicz, Tamás; Somogyvári-Vigh, Aniko; Arimura, Akira.

In: Regulatory Peptides, Vol. 78, No. 1-3, 30.11.1998, p. 83-88.

Research output: Contribution to journalArticle

@article{6bebb9b55ccb4587a48f857c915a2a2f,
title = "Immunohistochemical demonstration of the intracellular localization of pituitary adenylate cyclase activating polypeptide-like immunoreactivity in the rat testis using the stamp preparation",
abstract = "Radioimmunoassay has revealed an abundance of PACAP in the rat testis. In the present study, a novel stamp preparation together with light microscopic immunohistochemistry were used to investigate in detail the intracellular localization of PACAP-like immunoreactivity (PACAP-LI) in rat germ cells. Samples were obtained by pressing the freshly cut surfaces of the testes against glass slides. PACAP-LI was clearly identified in developing acrosomes using five antisera which recognize PACAP 38, or both PACAP 27 and PACAP 38. Immunoreactivity with the antisera specific to PACAP38 was strictly localized in the developing acrosomes of the spermatids but vanished in the mature spermatids. Using an antiserum which detects PACAP 27 specifically, little staining was observed. Based on the specificities of antisera used, it was suggested that the PACAP-LI in the acrosomes represents mainly PACAP 38-LI. In addition, the present results supported the usefulness of the stamp preparation for immunohistochemical study of testicular tissues. Copyright (C) 1998 Elsevier Science B.V.",
keywords = "Immunohistochemistry, PACAP, Rat, Stamp preparation, Testis",
author = "Hitoshi Yanaihara and S{\'a}ndor Vigh and Tam{\'a}s Kozicz and Aniko Somogyv{\'a}ri-Vigh and Akira Arimura",
year = "1998",
month = "11",
day = "30",
doi = "10.1016/S0167-0115(98)00125-6",
language = "English",
volume = "78",
pages = "83--88",
journal = "Regulatory Peptides",
issn = "0167-0115",
publisher = "Elsevier",
number = "1-3",

}

TY - JOUR

T1 - Immunohistochemical demonstration of the intracellular localization of pituitary adenylate cyclase activating polypeptide-like immunoreactivity in the rat testis using the stamp preparation

AU - Yanaihara, Hitoshi

AU - Vigh, Sándor

AU - Kozicz, Tamás

AU - Somogyvári-Vigh, Aniko

AU - Arimura, Akira

PY - 1998/11/30

Y1 - 1998/11/30

N2 - Radioimmunoassay has revealed an abundance of PACAP in the rat testis. In the present study, a novel stamp preparation together with light microscopic immunohistochemistry were used to investigate in detail the intracellular localization of PACAP-like immunoreactivity (PACAP-LI) in rat germ cells. Samples were obtained by pressing the freshly cut surfaces of the testes against glass slides. PACAP-LI was clearly identified in developing acrosomes using five antisera which recognize PACAP 38, or both PACAP 27 and PACAP 38. Immunoreactivity with the antisera specific to PACAP38 was strictly localized in the developing acrosomes of the spermatids but vanished in the mature spermatids. Using an antiserum which detects PACAP 27 specifically, little staining was observed. Based on the specificities of antisera used, it was suggested that the PACAP-LI in the acrosomes represents mainly PACAP 38-LI. In addition, the present results supported the usefulness of the stamp preparation for immunohistochemical study of testicular tissues. Copyright (C) 1998 Elsevier Science B.V.

AB - Radioimmunoassay has revealed an abundance of PACAP in the rat testis. In the present study, a novel stamp preparation together with light microscopic immunohistochemistry were used to investigate in detail the intracellular localization of PACAP-like immunoreactivity (PACAP-LI) in rat germ cells. Samples were obtained by pressing the freshly cut surfaces of the testes against glass slides. PACAP-LI was clearly identified in developing acrosomes using five antisera which recognize PACAP 38, or both PACAP 27 and PACAP 38. Immunoreactivity with the antisera specific to PACAP38 was strictly localized in the developing acrosomes of the spermatids but vanished in the mature spermatids. Using an antiserum which detects PACAP 27 specifically, little staining was observed. Based on the specificities of antisera used, it was suggested that the PACAP-LI in the acrosomes represents mainly PACAP 38-LI. In addition, the present results supported the usefulness of the stamp preparation for immunohistochemical study of testicular tissues. Copyright (C) 1998 Elsevier Science B.V.

KW - Immunohistochemistry

KW - PACAP

KW - Rat

KW - Stamp preparation

KW - Testis

UR - http://www.scopus.com/inward/record.url?scp=0032583177&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032583177&partnerID=8YFLogxK

U2 - 10.1016/S0167-0115(98)00125-6

DO - 10.1016/S0167-0115(98)00125-6

M3 - Article

VL - 78

SP - 83

EP - 88

JO - Regulatory Peptides

JF - Regulatory Peptides

SN - 0167-0115

IS - 1-3

ER -