Immunohistochemical demonstration of a novel hypothalamic peptide, pituitary adenylate cyclase- activating polypeptide, in the ovine hypothalamus

Katalin Köves, Akira Arimura, Anikó Somogyvári-Vigh, S. Vígh, Jim Miller, Akira Arimura

Research output: Contribution to journalArticle

231 Citations (Scopus)

Abstract

We recently reported isolation, characterization and synthesis of a novel ovine hypothalamic peptide with 38 residues which stimulates accumulation of cAMP in rat anterior pituitary cell cultures. The peptide was named PACAP38 (pituitary adenylate cyclase-activating polypeptide with 38 residues). The presence of another peptide corresponding to the N- terminal 1-27 residues (PACAP27) was also demonstrated. Both PACAP38 and PACAP27 have an amidated C-terminus. Antisera against synthetic PACAP27 were generated in rabbits. These antisera were tested for titer and specificity in enzyme- linked immunosorbent assay. One of the antisera (no. 88121-3) exhibited a high titer of antibody, which was specific to PACAP27 and PACAP38 with exception of slight cross-reactivity with ovine CRF.

Original languageEnglish
Pages (from-to)264-271
Number of pages8
JournalEndocrinology
Volume127
Issue number1
DOIs
Publication statusPublished - Jul 1990

ASJC Scopus subject areas

  • Endocrinology

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