Immunohistochemical Characterization of Reactive Epithelial Changes in Odontogenic Keratocysts

Dorottya Cserni, Tamás Zombori, Anette Stájer, Annamária Rimovszki, Gábor Cserni, Zoltán Baráth

Research output: Contribution to journalArticle

Abstract

Odontogenic keratocysts (OKCs) have a diagnostic thin epithelial lining characterised by a linear epithelial connective tissue interface generally lacking inflammatory changes, basal palisading of the nuclei and a wavy parakeratotic layer on the surface. This typical epithelium may convert to a thicker non-keratinizing one with rete pegs and a relatively flat surface after operative decompression. The aim was to characterize this type of epithelial change by immunohistochemistry for bcl2, keratin17, 10 and 19. Eleven out of 33 archived OKCs demonstrated an altered epithelium related to previous biopsy, decompressing drainage or inflammation. The typical basal bcl2 staining was lost in 10/11 cases; transepithelial CK17 was lost or markedly reduced in 9/11 cases. CK10 displayed a segmental upper layer staining in OKCs, and its loss or partial loss in the altered epithelium did not differ from negative areas of OKCs. CK19 displayed various staining patterns in the altered epithelium from lost to maintained in a patchy transepithelial distribution, the latter of which did not differ from the typical OKC staining pattern. Three of four non-keratinizing epithelial linings with basal palisading displayed immunostaining reminiscent of typical OKC epithelium. The lack of a typical epithelium is not sufficient to exclude the diagnosis of OKC if the sampling is not generous (e.g. biopsy), and the presence of non-keratinizing epithelium with basal palisading and an immunophenotype characteristic of OKC (basal bcl2, patchy or diffuse CK17 and upper layer CK10 positivity) may be consistent with the OKC diagnosis even in the absence of typical epithelial lining.

Original languageEnglish
JournalPathology and Oncology Research
DOIs
Publication statusAccepted/In press - Jan 1 2019

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Odontogenic Cysts
Epithelium
Staining and Labeling
Biopsy
Decompression
Basal Ganglia
Connective Tissue
Drainage
Immunohistochemistry
Inflammation

Keywords

  • bcl2
  • Cytokeratin 10
  • Cytokeratin 17
  • Cytokeratin 19
  • Immunohistochemistry
  • Odontogenic keratocyst

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Oncology
  • Cancer Research

Cite this

Immunohistochemical Characterization of Reactive Epithelial Changes in Odontogenic Keratocysts. / Cserni, Dorottya; Zombori, Tamás; Stájer, Anette; Rimovszki, Annamária; Cserni, Gábor; Baráth, Zoltán.

In: Pathology and Oncology Research, 01.01.2019.

Research output: Contribution to journalArticle

Cserni, Dorottya ; Zombori, Tamás ; Stájer, Anette ; Rimovszki, Annamária ; Cserni, Gábor ; Baráth, Zoltán. / Immunohistochemical Characterization of Reactive Epithelial Changes in Odontogenic Keratocysts. In: Pathology and Oncology Research. 2019.
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abstract = "Odontogenic keratocysts (OKCs) have a diagnostic thin epithelial lining characterised by a linear epithelial connective tissue interface generally lacking inflammatory changes, basal palisading of the nuclei and a wavy parakeratotic layer on the surface. This typical epithelium may convert to a thicker non-keratinizing one with rete pegs and a relatively flat surface after operative decompression. The aim was to characterize this type of epithelial change by immunohistochemistry for bcl2, keratin17, 10 and 19. Eleven out of 33 archived OKCs demonstrated an altered epithelium related to previous biopsy, decompressing drainage or inflammation. The typical basal bcl2 staining was lost in 10/11 cases; transepithelial CK17 was lost or markedly reduced in 9/11 cases. CK10 displayed a segmental upper layer staining in OKCs, and its loss or partial loss in the altered epithelium did not differ from negative areas of OKCs. CK19 displayed various staining patterns in the altered epithelium from lost to maintained in a patchy transepithelial distribution, the latter of which did not differ from the typical OKC staining pattern. Three of four non-keratinizing epithelial linings with basal palisading displayed immunostaining reminiscent of typical OKC epithelium. The lack of a typical epithelium is not sufficient to exclude the diagnosis of OKC if the sampling is not generous (e.g. biopsy), and the presence of non-keratinizing epithelium with basal palisading and an immunophenotype characteristic of OKC (basal bcl2, patchy or diffuse CK17 and upper layer CK10 positivity) may be consistent with the OKC diagnosis even in the absence of typical epithelial lining.",
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