Immunohistochemical assessment and prognostic value of hepatitis B virus X protein in chronic hepatitis and primary hepatocellular carcinomas using anti-HBxAg monoclonal antibody

J. Pál, Csilla Somogyi, Ágnes Szmolenszky, György Szekeres, József Sípos, Géza Hegedüs, Ilona Martzinovits, János Molnár, P. Németh

Research output: Contribution to journalArticle

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Abstract

Hepatitis B virus (HBV) is the most meaningful risk factor in chronic hepatitis, cirrhosis and primary hepatocellular carcinoma (PHC). The hepatitis B virus X protein (HBxAg) is a multifunctional protein with many important functions in hepatocellular carcinogenesis. A monoclonal anti-HBxAg antibody was developed in our laboratory and characterized by different methods. Using this antibody HBxAg was detected in formaldehyde fixed paraffin embedded tissue sections of 72 liver biopsies from patients with acute hepatitis, chronic hepatitis, cirrhosis and primary hepatocellular carcinoma. The co-expression of hepatitis B surface antigen (HBsAg), hepatitis B core antigen (HBcAg) and HBxAg was compared. The histological and cytological localization of the detected HBxAg showed a characteristic distribution in different stages of HBV infection. Strong and diffuse nuclear reaction was detected in PHC cases in contrast to the focal, cytoplasmic and nuclear labeling in the acute and chronic B hepatitis cases. Our antibody seems to be a suitable prognostic marker for routine pathohistological diagnosis and for comparative pathological and epidemiological research on the develop ment of PHC.

Original languageEnglish
Pages (from-to)178-184
Number of pages7
JournalPathology and Oncology Research
Volume7
Issue number3
Publication statusPublished - 2001

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Chronic Hepatitis
Hepatocellular Carcinoma
Monoclonal Antibodies
Hepatitis B virus
Fibrosis
Hepatitis B Core Antigens
Antibodies
Chronic Hepatitis B
Virus Diseases
Hepatitis B Surface Antigens
Paraffin
Formaldehyde
Hepatitis
Anti-Idiotypic Antibodies
Carcinogenesis
Biopsy
hepatitis B virus X protein
Liver
Research
Proteins

Keywords

  • Chronic B virus hepatitis
  • Hepatitis-B X protein
  • Immunohistochemistry
  • Monoclonal anti-hbx antibody
  • Primary hepatocellular carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

Immunohistochemical assessment and prognostic value of hepatitis B virus X protein in chronic hepatitis and primary hepatocellular carcinomas using anti-HBxAg monoclonal antibody. / Pál, J.; Somogyi, Csilla; Szmolenszky, Ágnes; Szekeres, György; Sípos, József; Hegedüs, Géza; Martzinovits, Ilona; Molnár, János; Németh, P.

In: Pathology and Oncology Research, Vol. 7, No. 3, 2001, p. 178-184.

Research output: Contribution to journalArticle

Pál, J. ; Somogyi, Csilla ; Szmolenszky, Ágnes ; Szekeres, György ; Sípos, József ; Hegedüs, Géza ; Martzinovits, Ilona ; Molnár, János ; Németh, P. / Immunohistochemical assessment and prognostic value of hepatitis B virus X protein in chronic hepatitis and primary hepatocellular carcinomas using anti-HBxAg monoclonal antibody. In: Pathology and Oncology Research. 2001 ; Vol. 7, No. 3. pp. 178-184.
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