Immunohistochemical analysis of cytokeratin 7 expression in resting and proliferating biliary structures of rat liver

S. Paku, Katalin Dezso, L. Kópper, P. Nagy

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39 Citations (Scopus)

Abstract

Cytokeratins are the largest subfamily of intermediate filament proteins and include more than 20 different gene products, which are expressed in an epithelial tissue-specific manner. We studied by immunohistochemistry and confocal microscopy the distribution of cytokeratin subtypes in the biliary system of adult rat liver. A cytokeratin (CK)19+/7- cholangiocyte population was observed in the smaller branches of the biliary tree including the canals of Hering. They proliferated after 2-acetaminofluorene (AAF) administration, although later the typical oval cells expressed CK7. This observation suggests that cholangiocytes with this cytokeratin phenotype may harbor adult hepatic stem cells. The CK19+/7- cholangiocytes were not present in the rat liver at birth, but developed postnatally. Similar cell populations were not observed in human livers. In conclusion, we propose that the CK19+/7- phenotype may be characteristic for adult hepatic stem cells in rat liver and that these cells are generated de novo after birth.

Original languageEnglish
Pages (from-to)863-870
Number of pages8
JournalHepatology
Volume42
Issue number4
DOIs
Publication statusPublished - Oct 2005

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Keratin-7
Keratins
Adult Stem Cells
Liver
Biliary Tract
Hepatocytes
Parturition
Keratin-19
Phenotype
Intermediate Filament Proteins
Confocal Microscopy
Population
Epithelium
Immunohistochemistry
Genes

ASJC Scopus subject areas

  • Hepatology

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Immunohistochemical analysis of cytokeratin 7 expression in resting and proliferating biliary structures of rat liver. / Paku, S.; Dezso, Katalin; Kópper, L.; Nagy, P.

In: Hepatology, Vol. 42, No. 4, 10.2005, p. 863-870.

Research output: Contribution to journalArticle

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