Immunogold localisation of ubiquitin-protein conjugates in Sf9 insect cells. Implications for the biogenesis of lysosome-related organelles

P. Lőw, Fergus J. Doherty, M. Sass, J. Kovács, R. John Mayer, L. László

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Immunogold electron microscopy with antibodies which primarily detect ubiquitin-protein conjugates shows conjugate-specific gold particles enriched severalfold in acid phosphatase-positive lysosomes and multivesicular bodies in insect Sf9 cells. The observations demonstrate that ubiquitinated proteins are associated with small acid phosphatase-containing primary lysosomes (transport vesicles) and indicate a pathway in which primary lysosomes fuse with multivesicular bodies to generate mature lysosome-related structures.

Original languageEnglish
Pages (from-to)152-156
Number of pages5
JournalFEBS Letters
Volume316
Issue number2
DOIs
Publication statusPublished - Jan 25 1993

Fingerprint

Sf9 Cells
Acid Phosphatase
Ubiquitin
Lysosomes
Organelles
Insects
Ubiquitinated Proteins
Multivesicular Bodies
Electric fuses
Gold
Electron microscopy
Proteins
Transport Vesicles
Antibodies
Electron Microscopy

Keywords

  • 20-Hydroxyecdysone
  • Lysosome, acid phosphatase
  • Sf9 insect cell
  • Ubiquitin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Immunogold localisation of ubiquitin-protein conjugates in Sf9 insect cells. Implications for the biogenesis of lysosome-related organelles. / Lőw, P.; Doherty, Fergus J.; Sass, M.; Kovács, J.; Mayer, R. John; László, L.

In: FEBS Letters, Vol. 316, No. 2, 25.01.1993, p. 152-156.

Research output: Contribution to journalArticle

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