Immunoglobulin variable regions usage by B-lymphocytes infiltrating a human breast medullary carcinoma

Beatrix Kotlan, Nadège Gruel, Brigitte Zafrani, Gabrielle Füredi, Janos Foldi, Gyoso G. Petranyi, Wolf Herman Fridman, Jean Luc Teillaud

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Breast medullary carcinoma are heavily infiltrated by B-lymphocytes and associated with a good prognosis despite their high histological grade. We investigated the Ig repertoire of B-lymphocytes infiltrating one such tumour. A single cell suspension was obtained from a tumor specimen by enzymatic digestion. VH, Vκ, and Vλ regions were amplified by RT-PCR using mixtures of primers optimized to maximize the diversity of the PCR products. They were then cloned and sequenced. Analysis of 9 VH, 5 Vκ, and 10 Vλ sequences using the Kabat database indicated that several VH and VL region subgroups (I, II and III) are expressed by B-lymphocytes infiltrating this tumor. The analysis of CDR3 regions also showed a variability, although some VH and VL clones exhibited identical or nearly identical sequences. Thus, the B-cell infiltration observed in this breast medullary carcinoma does not reflect a monoclonal proliferation and represents an oligoclonal or a polyclonal B- cell proliferation.

Original languageEnglish
Pages (from-to)143-151
Number of pages9
JournalImmunology letters
Volume65
Issue number3
DOIs
Publication statusPublished - Feb 1 1999

Keywords

  • B-lymphocytes
  • Human variable regions
  • Medullary breast carcinoma

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Kotlan, B., Gruel, N., Zafrani, B., Füredi, G., Foldi, J., Petranyi, G. G., Fridman, W. H., & Teillaud, J. L. (1999). Immunoglobulin variable regions usage by B-lymphocytes infiltrating a human breast medullary carcinoma. Immunology letters, 65(3), 143-151. https://doi.org/10.1016/S0165-2478(98)00098-4