Immunoglobulin G from patients with antiphospholipid syndrome impairs the fibrin dissolution with plasmin

K. Kolev, Judit Gombás, Balázs Váradi, Judit Skopál, Katalin Mede, Ervin Pitlik, Zoltán Nagy, R. Machovich

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Immunoglobulin G (IgG) isolated from normal human blood plasma stabilizes the structure of perfused crosslinked fibrin and prolongs the time for its dissolution with plasmin, when the fibrin surface is exposed to 500 s-1 shear rate flow. The IgG from patients suffering in antiphospholipid syndrome with thrombotic complications exerts even stronger antifibrinolytic effect. A patient, whose IgG does not affect the fibrin dissolution with plasmin, displays a bleeding tendency. The shear stress-induced disassembly of the fibrin clots containing IgGs with antifibrinolytic potency occurs at a much more advanced stage of fibrin digestion, as evidenced by the electrophoretic pattern of the urea-treated samples. The antifibrinolytic effects are also produced under static conditions and these are caused by the variable portion of the IgG molecules (fragment Fab), whereas the constant part (fragment Fc) has no inhibitory effect. The IgGs with antifibrinolytic properties do not affect directly the plasmin activity in amidolytic assay, but the IgGs from APS patients obliterate the competition of the fibrin and the peptidyl-p-nitroanilide for the protease in the same assay system suggesting interference of the IgGs with the plasmin action on the fibrin substrate. Thus, the correlation of the clinical symptoms with the effect of the isolated IgG on the dissolution of perfused fibrin clots supports a physiological and a pathological role of IgG in the fibrinolytic process related to the variability of the cross-reactions of immunoglobulins with fibrin, fibrin degradation products or fibrin-plasmin complexes.

Original languageEnglish
Pages (from-to)502-508
Number of pages7
JournalThrombosis and Haemostasis
Volume87
Issue number3
Publication statusPublished - 2002

Fingerprint

Antiphospholipid Syndrome
Fibrinolysin
Fibrin
Immunoglobulin G
Antifibrinolytic Agents
Fibrin Fibrinogen Degradation Products
Immunoglobulin Fab Fragments
Cross Reactions
Urea
Immunoglobulins
Digestion
Peptide Hydrolases
Hemorrhage

Keywords

  • Antiphospholipid syndrome
  • Fibrin
  • Fibrinolysis
  • Immunoglobulin G
  • Plasmin

ASJC Scopus subject areas

  • Hematology

Cite this

Immunoglobulin G from patients with antiphospholipid syndrome impairs the fibrin dissolution with plasmin. / Kolev, K.; Gombás, Judit; Váradi, Balázs; Skopál, Judit; Mede, Katalin; Pitlik, Ervin; Nagy, Zoltán; Machovich, R.

In: Thrombosis and Haemostasis, Vol. 87, No. 3, 2002, p. 502-508.

Research output: Contribution to journalArticle

Kolev, K, Gombás, J, Váradi, B, Skopál, J, Mede, K, Pitlik, E, Nagy, Z & Machovich, R 2002, 'Immunoglobulin G from patients with antiphospholipid syndrome impairs the fibrin dissolution with plasmin', Thrombosis and Haemostasis, vol. 87, no. 3, pp. 502-508.
Kolev, K. ; Gombás, Judit ; Váradi, Balázs ; Skopál, Judit ; Mede, Katalin ; Pitlik, Ervin ; Nagy, Zoltán ; Machovich, R. / Immunoglobulin G from patients with antiphospholipid syndrome impairs the fibrin dissolution with plasmin. In: Thrombosis and Haemostasis. 2002 ; Vol. 87, No. 3. pp. 502-508.
@article{65548715386f4a7c9cb5418cac50e618,
title = "Immunoglobulin G from patients with antiphospholipid syndrome impairs the fibrin dissolution with plasmin",
abstract = "Immunoglobulin G (IgG) isolated from normal human blood plasma stabilizes the structure of perfused crosslinked fibrin and prolongs the time for its dissolution with plasmin, when the fibrin surface is exposed to 500 s-1 shear rate flow. The IgG from patients suffering in antiphospholipid syndrome with thrombotic complications exerts even stronger antifibrinolytic effect. A patient, whose IgG does not affect the fibrin dissolution with plasmin, displays a bleeding tendency. The shear stress-induced disassembly of the fibrin clots containing IgGs with antifibrinolytic potency occurs at a much more advanced stage of fibrin digestion, as evidenced by the electrophoretic pattern of the urea-treated samples. The antifibrinolytic effects are also produced under static conditions and these are caused by the variable portion of the IgG molecules (fragment Fab), whereas the constant part (fragment Fc) has no inhibitory effect. The IgGs with antifibrinolytic properties do not affect directly the plasmin activity in amidolytic assay, but the IgGs from APS patients obliterate the competition of the fibrin and the peptidyl-p-nitroanilide for the protease in the same assay system suggesting interference of the IgGs with the plasmin action on the fibrin substrate. Thus, the correlation of the clinical symptoms with the effect of the isolated IgG on the dissolution of perfused fibrin clots supports a physiological and a pathological role of IgG in the fibrinolytic process related to the variability of the cross-reactions of immunoglobulins with fibrin, fibrin degradation products or fibrin-plasmin complexes.",
keywords = "Antiphospholipid syndrome, Fibrin, Fibrinolysis, Immunoglobulin G, Plasmin",
author = "K. Kolev and Judit Gomb{\'a}s and Bal{\'a}zs V{\'a}radi and Judit Skop{\'a}l and Katalin Mede and Ervin Pitlik and Zolt{\'a}n Nagy and R. Machovich",
year = "2002",
language = "English",
volume = "87",
pages = "502--508",
journal = "Thrombosis and Haemostasis",
issn = "0340-6245",
publisher = "Schattauer GmbH",
number = "3",

}

TY - JOUR

T1 - Immunoglobulin G from patients with antiphospholipid syndrome impairs the fibrin dissolution with plasmin

AU - Kolev, K.

AU - Gombás, Judit

AU - Váradi, Balázs

AU - Skopál, Judit

AU - Mede, Katalin

AU - Pitlik, Ervin

AU - Nagy, Zoltán

AU - Machovich, R.

PY - 2002

Y1 - 2002

N2 - Immunoglobulin G (IgG) isolated from normal human blood plasma stabilizes the structure of perfused crosslinked fibrin and prolongs the time for its dissolution with plasmin, when the fibrin surface is exposed to 500 s-1 shear rate flow. The IgG from patients suffering in antiphospholipid syndrome with thrombotic complications exerts even stronger antifibrinolytic effect. A patient, whose IgG does not affect the fibrin dissolution with plasmin, displays a bleeding tendency. The shear stress-induced disassembly of the fibrin clots containing IgGs with antifibrinolytic potency occurs at a much more advanced stage of fibrin digestion, as evidenced by the electrophoretic pattern of the urea-treated samples. The antifibrinolytic effects are also produced under static conditions and these are caused by the variable portion of the IgG molecules (fragment Fab), whereas the constant part (fragment Fc) has no inhibitory effect. The IgGs with antifibrinolytic properties do not affect directly the plasmin activity in amidolytic assay, but the IgGs from APS patients obliterate the competition of the fibrin and the peptidyl-p-nitroanilide for the protease in the same assay system suggesting interference of the IgGs with the plasmin action on the fibrin substrate. Thus, the correlation of the clinical symptoms with the effect of the isolated IgG on the dissolution of perfused fibrin clots supports a physiological and a pathological role of IgG in the fibrinolytic process related to the variability of the cross-reactions of immunoglobulins with fibrin, fibrin degradation products or fibrin-plasmin complexes.

AB - Immunoglobulin G (IgG) isolated from normal human blood plasma stabilizes the structure of perfused crosslinked fibrin and prolongs the time for its dissolution with plasmin, when the fibrin surface is exposed to 500 s-1 shear rate flow. The IgG from patients suffering in antiphospholipid syndrome with thrombotic complications exerts even stronger antifibrinolytic effect. A patient, whose IgG does not affect the fibrin dissolution with plasmin, displays a bleeding tendency. The shear stress-induced disassembly of the fibrin clots containing IgGs with antifibrinolytic potency occurs at a much more advanced stage of fibrin digestion, as evidenced by the electrophoretic pattern of the urea-treated samples. The antifibrinolytic effects are also produced under static conditions and these are caused by the variable portion of the IgG molecules (fragment Fab), whereas the constant part (fragment Fc) has no inhibitory effect. The IgGs with antifibrinolytic properties do not affect directly the plasmin activity in amidolytic assay, but the IgGs from APS patients obliterate the competition of the fibrin and the peptidyl-p-nitroanilide for the protease in the same assay system suggesting interference of the IgGs with the plasmin action on the fibrin substrate. Thus, the correlation of the clinical symptoms with the effect of the isolated IgG on the dissolution of perfused fibrin clots supports a physiological and a pathological role of IgG in the fibrinolytic process related to the variability of the cross-reactions of immunoglobulins with fibrin, fibrin degradation products or fibrin-plasmin complexes.

KW - Antiphospholipid syndrome

KW - Fibrin

KW - Fibrinolysis

KW - Immunoglobulin G

KW - Plasmin

UR - http://www.scopus.com/inward/record.url?scp=0036125784&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036125784&partnerID=8YFLogxK

M3 - Article

C2 - 11916083

AN - SCOPUS:0036125784

VL - 87

SP - 502

EP - 508

JO - Thrombosis and Haemostasis

JF - Thrombosis and Haemostasis

SN - 0340-6245

IS - 3

ER -