Immunogenicity of the S protein of transmissible gastroenteritis virus expressed in baculovirus

T. Tuboly, E. Nagy, J. R. Dennis, J. B. Derbyshire

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Seven fragments of the spike (S) gene cDNA of transmissible gastroenteritis virus (TGEV), as well as the full length cDNA, were cloned and expressed in baculovirus vectors. Piglets were immunized with cells infected with the recombinant viruses. Each of the recombinants induced TGEV-specific antibodies detected in a fixed cell enzyme immunoassay. The amino terminal half of the S protein, containing all four major antigenic sites (A, B, C and D), and encoded by a 2.2 kb fragment of the S gene, induced virus neutralizing (VN) antibody titers comparable with those induced by the complete S protein. Recombinant proteins lacking the A antigenic site, or with a deletion including the putative receptor binding sites and the D antigenic site, were not capable of inducing levels of VN antibodies similar to those induced by the whole S protein.

Original languageEnglish
Pages (from-to)55-67
Number of pages13
JournalArchives of Virology
Volume137
Issue number1-2
DOIs
Publication statusPublished - Mar 1994

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Transmissible gastroenteritis virus
Baculoviridae
Protein S
Viruses
Neutralizing Antibodies
Complementary DNA
Immunoenzyme Techniques
Recombinant Proteins
Genes
Binding Sites
Antibodies

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

Immunogenicity of the S protein of transmissible gastroenteritis virus expressed in baculovirus. / Tuboly, T.; Nagy, E.; Dennis, J. R.; Derbyshire, J. B.

In: Archives of Virology, Vol. 137, No. 1-2, 03.1994, p. 55-67.

Research output: Contribution to journalArticle

Tuboly, T. ; Nagy, E. ; Dennis, J. R. ; Derbyshire, J. B. / Immunogenicity of the S protein of transmissible gastroenteritis virus expressed in baculovirus. In: Archives of Virology. 1994 ; Vol. 137, No. 1-2. pp. 55-67.
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