Immunogenicity of porcine transmissible gastroenteritis virus spike protein expressed in plants

T. Tuboly, W. Yu, A. Bailey, S. Degrandis, S. Du, L. Erickson, É Nagy

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Transgenic plants expressing recombinant proteins from pathogenic microorganisms provide an inexpensive edible vaccine for induction of local immunity. Three transgenic plant lines were generated expressing the spike (S) protein of transmissible gastroenteritis virus (TGEV), a protein crucial for establishing mucosal immunity. All three of them were driven by a strong plant promoter. One construct contained the 3.7 kb 5' end of the native S gene sequence. In the second construct part of the S gene, from nucleotide 49 to 1785, was modified for optimal plant recognition and was fused to a plant signal peptide coding sequence. The third construct contained the D epitope-coding region of the S gene, from nucleotide 1201 to 1591, which was fused to the alfalfa beta-amylase gene. The S gene products were detected by enzyme-linked immunosorbent assay (ELISA) and Western blotting. Antigens from all three transgenic plant lines induced TGEV-specific immune responses in pigs as determined by virus neutralization and ELISA, and the resultant antibody titers for all three constructs were similar. Copyright (C) 2000 Elsevier Science Ltd.

Original languageEnglish
Pages (from-to)2023-2028
Number of pages6
JournalVaccine
Volume18
Issue number19
DOIs
Publication statusPublished - Apr 3 2000

Keywords

  • Oral vaccine
  • TGEV
  • Transgenic plant

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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