Immunogene expression in head kidney and spleen of common carp (Cyprinus carpio L.) following thermal stress and challenge with Gram-negative bacterium, Aeromonas hydrophila

Neetu Shahi, László Ardó, Gyöngyvér Fazekas, Elen Gócza, Shivendra Kumar, Norbert Rèvèsz, Zsuzsanna Jakabné Sándor, Zsuzsanna Molnár, G. Jeney, Z. Jeney

Research output: Contribution to journalArticle

Abstract

To evaluate the effect of thermal and microbial stress on the immune response of common carp (Cyprinus carpio L.), relative mRNA expression level of pro-inflammatory cytokines [tumor necrosis factor alpha (TNF-α) and interleukin (IL)-1β] and other genes related to immune or stress response [inducible nitric oxide synthase (iNOS), heat shock protein 70 (Hsp70), superoxide dismutase one (SOD1), and glucocorticoid receptor (GR)] was measured by quantitative PCR (qPCR). In addition, total protein and total immunoglobulin level in blood plasma of experimental common carp was also assayed. All the above parameters were estimated 24 h post-challenge with Gram-negative bacterium, Aeromonas hydrophila. Common carp (54.89 ± 6.90 g) were initially exposed to 20 °C (control group) and 30 °C (thermal stress group) water temperature for 30 days, followed by experimental challenge with 2.29 × 108 colony forming unit/mL (CFU/mL; LD50 dose) of A. hydrophila. Exposure of fish to thermal stress and subsequently challenge with A. hydrophila significantly (P < 0.05) increases the IL-1β mRNA expression in head kidney and spleen of common carp by ~ 39.94 and ~ 4.11-fold, respectively. However, TNF-α mRNA expression in spleen decreased ~ 5.63-fold in control fish challenged with A. hydrophila. Thermal stress and challenge with bacterium suppresses the iNOS and GR mRNA expression in spleen of common carp. Moreover, significant (P < 0.05) increase in total protein content of blood plasma (~ 43 mg/g) was evident in fish exposed to thermal stress and challenged with A. hydrophila. In conclusion, our study highlights the importance of elevated temperature stress and microbial infection in differential regulation of expression of several immunogenes in common carp.

Original languageEnglish
Pages (from-to)1-15
Number of pages15
JournalAquaculture International
DOIs
Publication statusAccepted/In press - Feb 12 2018

Fingerprint

Aeromonas hydrophila
thermal stress
Cyprinus carpio
Gram-negative bacteria
spleen
kidneys
bacterium
nitric oxide
tumor
interleukin-1
protein
blood plasma
tumor necrosis factor-alpha
fish
blood
fold
plasma
immune response
heat shock
lethal dose 50

Keywords

  • Aeromonas hydrophila
  • Common carp
  • Cytokines
  • Quantitative PCR
  • Thermal stress

ASJC Scopus subject areas

  • Aquatic Science
  • Agronomy and Crop Science

Cite this

Immunogene expression in head kidney and spleen of common carp (Cyprinus carpio L.) following thermal stress and challenge with Gram-negative bacterium, Aeromonas hydrophila. / Shahi, Neetu; Ardó, László; Fazekas, Gyöngyvér; Gócza, Elen; Kumar, Shivendra; Rèvèsz, Norbert; Sándor, Zsuzsanna Jakabné; Molnár, Zsuzsanna; Jeney, G.; Jeney, Z.

In: Aquaculture International, 12.02.2018, p. 1-15.

Research output: Contribution to journalArticle

Shahi, Neetu ; Ardó, László ; Fazekas, Gyöngyvér ; Gócza, Elen ; Kumar, Shivendra ; Rèvèsz, Norbert ; Sándor, Zsuzsanna Jakabné ; Molnár, Zsuzsanna ; Jeney, G. ; Jeney, Z. / Immunogene expression in head kidney and spleen of common carp (Cyprinus carpio L.) following thermal stress and challenge with Gram-negative bacterium, Aeromonas hydrophila. In: Aquaculture International. 2018 ; pp. 1-15.
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abstract = "To evaluate the effect of thermal and microbial stress on the immune response of common carp (Cyprinus carpio L.), relative mRNA expression level of pro-inflammatory cytokines [tumor necrosis factor alpha (TNF-α) and interleukin (IL)-1β] and other genes related to immune or stress response [inducible nitric oxide synthase (iNOS), heat shock protein 70 (Hsp70), superoxide dismutase one (SOD1), and glucocorticoid receptor (GR)] was measured by quantitative PCR (qPCR). In addition, total protein and total immunoglobulin level in blood plasma of experimental common carp was also assayed. All the above parameters were estimated 24 h post-challenge with Gram-negative bacterium, Aeromonas hydrophila. Common carp (54.89 ± 6.90 g) were initially exposed to 20 °C (control group) and 30 °C (thermal stress group) water temperature for 30 days, followed by experimental challenge with 2.29 × 108 colony forming unit/mL (CFU/mL; LD50 dose) of A. hydrophila. Exposure of fish to thermal stress and subsequently challenge with A. hydrophila significantly (P < 0.05) increases the IL-1β mRNA expression in head kidney and spleen of common carp by ~ 39.94 and ~ 4.11-fold, respectively. However, TNF-α mRNA expression in spleen decreased ~ 5.63-fold in control fish challenged with A. hydrophila. Thermal stress and challenge with bacterium suppresses the iNOS and GR mRNA expression in spleen of common carp. Moreover, significant (P < 0.05) increase in total protein content of blood plasma (~ 43 mg/g) was evident in fish exposed to thermal stress and challenged with A. hydrophila. In conclusion, our study highlights the importance of elevated temperature stress and microbial infection in differential regulation of expression of several immunogenes in common carp.",
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AU - Ardó, László

AU - Fazekas, Gyöngyvér

AU - Gócza, Elen

AU - Kumar, Shivendra

AU - Rèvèsz, Norbert

AU - Sándor, Zsuzsanna Jakabné

AU - Molnár, Zsuzsanna

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