Immunofluorescent examination of the kidney post mortem

Z. Laszik, B. Iványi, J. Ormos

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

106 selected kidneys removed at autopsy were studied by direct immunofluorescence using polyvalent antisera against human immunoglobulins, light chains, complement fractions and fibrinogen. The immunofluorescence was a suitable method to solve differential diagnostic problems that arose at autopsy. The diagnostic value was the most obvious in cases of immunologically mediated renal diseases and in immunologically mediated systemic diseases involving the kidneys. Negative immunofluorescence findings were also useful to determine the pathogenesis of renal lesions, especially in vasculopathies. The immunofluorescence of postmortem material showed similar disturbances to that obtained with biopsy material. At various sites, especially in the tubulo-interstitum, additional electron microscopical study was sometimes needed to localise the immune deposits exactly. The fluorescent microscopical examination of frozen sections of kidney taken at necropsy turned out to be more adequate than the immunoperoxidase examination of formalin-fixed, paraffin-embedded sections.

Original languageEnglish
Pages (from-to)13-18
Number of pages6
JournalHistology and Histopathology
Volume2
Issue number1
Publication statusPublished - 1987

Fingerprint

Autopsy
Fluorescent Antibody Technique
Kidney
Immunoglobulin Light Chains
Direct Fluorescent Antibody Technique
Kidney Diseases
Frozen Sections
Paraffin
Fibrinogen
Formaldehyde
Immune Sera
Electrons
Biopsy

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy
  • Histology
  • Pathology and Forensic Medicine

Cite this

Immunofluorescent examination of the kidney post mortem. / Laszik, Z.; Iványi, B.; Ormos, J.

In: Histology and Histopathology, Vol. 2, No. 1, 1987, p. 13-18.

Research output: Contribution to journalArticle

Laszik, Z. ; Iványi, B. ; Ormos, J. / Immunofluorescent examination of the kidney post mortem. In: Histology and Histopathology. 1987 ; Vol. 2, No. 1. pp. 13-18.
@article{9d6ccdac413d452e8c9f5a28a1758665,
title = "Immunofluorescent examination of the kidney post mortem",
abstract = "106 selected kidneys removed at autopsy were studied by direct immunofluorescence using polyvalent antisera against human immunoglobulins, light chains, complement fractions and fibrinogen. The immunofluorescence was a suitable method to solve differential diagnostic problems that arose at autopsy. The diagnostic value was the most obvious in cases of immunologically mediated renal diseases and in immunologically mediated systemic diseases involving the kidneys. Negative immunofluorescence findings were also useful to determine the pathogenesis of renal lesions, especially in vasculopathies. The immunofluorescence of postmortem material showed similar disturbances to that obtained with biopsy material. At various sites, especially in the tubulo-interstitum, additional electron microscopical study was sometimes needed to localise the immune deposits exactly. The fluorescent microscopical examination of frozen sections of kidney taken at necropsy turned out to be more adequate than the immunoperoxidase examination of formalin-fixed, paraffin-embedded sections.",
author = "Z. Laszik and B. Iv{\'a}nyi and J. Ormos",
year = "1987",
language = "English",
volume = "2",
pages = "13--18",
journal = "Histology and Histopathology",
issn = "0213-3911",
publisher = "Histology and Histopathology",
number = "1",

}

TY - JOUR

T1 - Immunofluorescent examination of the kidney post mortem

AU - Laszik, Z.

AU - Iványi, B.

AU - Ormos, J.

PY - 1987

Y1 - 1987

N2 - 106 selected kidneys removed at autopsy were studied by direct immunofluorescence using polyvalent antisera against human immunoglobulins, light chains, complement fractions and fibrinogen. The immunofluorescence was a suitable method to solve differential diagnostic problems that arose at autopsy. The diagnostic value was the most obvious in cases of immunologically mediated renal diseases and in immunologically mediated systemic diseases involving the kidneys. Negative immunofluorescence findings were also useful to determine the pathogenesis of renal lesions, especially in vasculopathies. The immunofluorescence of postmortem material showed similar disturbances to that obtained with biopsy material. At various sites, especially in the tubulo-interstitum, additional electron microscopical study was sometimes needed to localise the immune deposits exactly. The fluorescent microscopical examination of frozen sections of kidney taken at necropsy turned out to be more adequate than the immunoperoxidase examination of formalin-fixed, paraffin-embedded sections.

AB - 106 selected kidneys removed at autopsy were studied by direct immunofluorescence using polyvalent antisera against human immunoglobulins, light chains, complement fractions and fibrinogen. The immunofluorescence was a suitable method to solve differential diagnostic problems that arose at autopsy. The diagnostic value was the most obvious in cases of immunologically mediated renal diseases and in immunologically mediated systemic diseases involving the kidneys. Negative immunofluorescence findings were also useful to determine the pathogenesis of renal lesions, especially in vasculopathies. The immunofluorescence of postmortem material showed similar disturbances to that obtained with biopsy material. At various sites, especially in the tubulo-interstitum, additional electron microscopical study was sometimes needed to localise the immune deposits exactly. The fluorescent microscopical examination of frozen sections of kidney taken at necropsy turned out to be more adequate than the immunoperoxidase examination of formalin-fixed, paraffin-embedded sections.

UR - http://www.scopus.com/inward/record.url?scp=0023143007&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023143007&partnerID=8YFLogxK

M3 - Article

C2 - 2980698

AN - SCOPUS:0023143007

VL - 2

SP - 13

EP - 18

JO - Histology and Histopathology

JF - Histology and Histopathology

SN - 0213-3911

IS - 1

ER -