Immunofluorescence demonstration of digoxin in human myocardial tissue and isolated rat cardiac myocytes with monoclonal antibodies

P. Sótónyi, E. Somogyi, J. Rajs, M. Sundberg, B. Andersson

Research output: Contribution to journalArticle

Abstract

The aim of the present investigation was to identify the morphological correlates of digoxin binding sites in human heart muscle tissue and isolated viable rat heart myocytes. Cardiac glycoside linked to myocardial cells was demonstrated by monoclonal digoxin specific antibody and by FITC-conjugated anti-mouse immunoglobin serum. This versatile immunofluorescence method can be used in diagnostic and experimental studies of cardiac glycoside binding.

Original languageEnglish
Pages (from-to)157-163
Number of pages7
JournalForensic Science International
Volume47
Issue number2
DOIs
Publication statusPublished - 1990

Fingerprint

Cardiac Glycosides
Digoxin
Cardiac Myocytes
Fluorescent Antibody Technique
Rats
Demonstrations
Monoclonal Antibodies
Tissue
Fluorescein-5-isothiocyanate
Muscle Cells
Muscle
Myocardium
Binding Sites
Muscles
Antibodies
Serum

Keywords

  • Digoxin
  • Immunofluorescence method
  • Myocardium

ASJC Scopus subject areas

  • Genetics
  • Analytical Chemistry
  • Pathology and Forensic Medicine
  • Psychiatry and Mental health

Cite this

Immunofluorescence demonstration of digoxin in human myocardial tissue and isolated rat cardiac myocytes with monoclonal antibodies. / Sótónyi, P.; Somogyi, E.; Rajs, J.; Sundberg, M.; Andersson, B.

In: Forensic Science International, Vol. 47, No. 2, 1990, p. 157-163.

Research output: Contribution to journalArticle

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