Immunocytochemical study on the intracellular localization of the type 2 glucocorticoid receptor in the rat brain

J. A M van Eekelen, J. Kiss, H. M. Westphal, E. R. de Kloet

Research output: Contribution to journalArticle

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Abstract

The localization of the glucocorticoid receptor (GR) (type 2) in the rat brain was studied with immunocytochemistry using a monoclonal antibody against the rat liver GR. Strong GR immunoreactivity (GR-ir) was observed in neurons of limbic and brainstem structures known to be associated with the stress-activated circuitry, which suggest that these sites are responsive to glucocorticoid feedback. The intracellular localization of GR-ir was examined in CA1 and CA2 pyramidal neurons of the hippocampus. In intact rats GR-ir is predominantly present in the cell nucleus. Adrenalectomy (ADX) caused a slow depletion of the GR-ir signal from the cell nucleus until near detection limits at two weeks postsurgery. At that time, 1 h after administration to longterm ADX rats the synthetic glucocorticoid (type 2) agonist RU 28362 as well as a moderate and high dose of corticosterone (CORT) markedly enhanced the cell nuclear Gr-ir. The type 2 antagonist RU 38486 also caused an increase of GR immunostaining in cell nuclei upon acute administration to ADX rats. The mineralocorticoid aldosterone (ALDO) and a low dose of CORT, which bind almost exclusively to type 1 corticosteroid receptors, were ineffective. In conclusion, our data suggest that in the hippocampal Ca1-2 neurons type 1 and type 2 corticosteroid receptors may coexist. The steroid-induced changes in cell nuclear immunoreactive GR staining intensity suggest possible cytoplasmic-cell nuclear translocation of GR and/or exposure of immunogenic GR domains.

Original languageEnglish
Pages (from-to)120-128
Number of pages9
JournalBrain Research
Volume436
Issue number1
DOIs
Publication statusPublished - Dec 8 1987

Fingerprint

Glucocorticoid Receptors
Brain
Cell Nucleus
Steroid Receptors
Corticosterone
Glucocorticoids
Neurons
Mifepristone
Mineralocorticoids
Pyramidal Cells
Adrenalectomy
Aldosterone
Brain Stem
Limit of Detection
Hippocampus
Immunohistochemistry
Steroids
Monoclonal Antibodies
Staining and Labeling
Liver

Keywords

  • Corticosteroid receptor
  • Immunocytochemistry
  • Rat brain

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Immunocytochemical study on the intracellular localization of the type 2 glucocorticoid receptor in the rat brain. / van Eekelen, J. A M; Kiss, J.; Westphal, H. M.; de Kloet, E. R.

In: Brain Research, Vol. 436, No. 1, 08.12.1987, p. 120-128.

Research output: Contribution to journalArticle

van Eekelen, J. A M ; Kiss, J. ; Westphal, H. M. ; de Kloet, E. R. / Immunocytochemical study on the intracellular localization of the type 2 glucocorticoid receptor in the rat brain. In: Brain Research. 1987 ; Vol. 436, No. 1. pp. 120-128.
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