Immunocytochemical identification of outer segment proteins in the rd chicken

Robert J. Ulshafer, Evelyn L. Clausnitzer, David M. Sherry, A. Szél, P. Röhlich

Research output: Contribution to journalArticle

Abstract

Immunoreactivities of two monoclonal antibodies (MAbs) that recognize cone photopigments were tested in the retinas of congenitally blind retinal degenerate (rd) chicks and compared to normally sighted carrier chicks, heterozygous for the mutation. MAb OS-2 had been previously determined to label rod and most cone outer segment membranes in normal chick retinas and is believed to bind to an epitope that is common to several photopigments in chickens. MAb COS-1 labels specifically middle-to-long-wavelength-sensitive cone photopigments in a number of vertebrate species. In rd chicks MAb OS-2 labeled the same number of rod outer segments at the same densities as carrier chicks. However, cone outer segments were less frequently and significantly less heavily labeled with this MAb at all ages tested (1 day, 1 week and 2 weeks post hatching). MAb COS-1 labeled the same number of cone outer segments in both rd and carrier retinas at 1 day of age, however, those outer segments that were labeled in rd specimens had significantly fewer gold particles on them. At both 1 week and 2 weeks of age, rd chick retinas had a significant reduction in numbers of cone outer segments labeled by COS-1. These findings support the hypothesis that the cone photopigment protein is abnormal in the rd chick model of hereditary blindness and retinal degeneration.

Original languageEnglish
Pages (from-to)209-216
Number of pages8
JournalExperimental Eye Research
Volume51
Issue number2
DOIs
Publication statusPublished - 1990

Fingerprint

Retina
Chickens
Proteins
Rod Cell Outer Segment
Retinal Degeneration
Vertebrate Photoreceptor Cells
Blindness
Gold
Vertebrates
Epitopes
Monoclonal Antibodies
Mutation
Membranes

Keywords

  • animal model
  • chicken
  • cone pigments
  • monoclonal antibodies
  • photoreceptor degeneration

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Immunocytochemical identification of outer segment proteins in the rd chicken. / Ulshafer, Robert J.; Clausnitzer, Evelyn L.; Sherry, David M.; Szél, A.; Röhlich, P.

In: Experimental Eye Research, Vol. 51, No. 2, 1990, p. 209-216.

Research output: Contribution to journalArticle

Ulshafer, Robert J. ; Clausnitzer, Evelyn L. ; Sherry, David M. ; Szél, A. ; Röhlich, P. / Immunocytochemical identification of outer segment proteins in the rd chicken. In: Experimental Eye Research. 1990 ; Vol. 51, No. 2. pp. 209-216.
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