Immunochemical detection of ochratoxin A in black Aspergillus strains

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177 Citations (Scopus)

Abstract

One hundred and fifty-seven strains belonging to Aspergillus section Nigri were tested for ochratoxin A production using three different methods: a relatively new immunochemical method based on an enzyme-linked immunosorbent assay (ELISA), thin-layer chromatography (TLC) and high- performance liquid chromatography (HPLC). The monoclonal antibody-based ELISA technique was successfully used to screen for low levels of ochratoxin A in the black Aspergilli without concentrating the culture filtrates. The results were confirmed by TLC and HPLC analysis and chemical derivatization. These latter methods required concentrated filtrates. Ochratoxin A was detected in the culture filtrates of five of the 12 A. carbonarius strains, none of the 45 A. japonicus strains and three of the 100 isolates in the A. niger aggregate (A. foetidus, A. awamori and A. niger).

Original languageEnglish
Pages (from-to)171-176
Number of pages6
JournalMycopathologia
Volume134
Issue number3
DOIs
Publication statusPublished - 1996

Keywords

  • Aspergillus awamori
  • Aspergillus carbonarius
  • Aspergillus foetidus
  • Aspergillus niger
  • ELISA
  • ochratoxin A

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Agronomy and Crop Science
  • veterinary (miscalleneous)

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