Immunization with a combination of ApoB and HSP60 epitopes significantly reduces early atherosclerotic lesion in Apobtm2SgyLdlrtm1Her/J mice

Xinjie Lu, Daxin Chen, V. Endrész, Min Xia, Ildiko Faludi, K. Burián, Andrea Szabo, Agnes Csanadi, A. Miczák, E. Gönczöl, Vijay Kakkar

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Objective: HSP60 is emerging as an immunodominant target of autoantibodies in atherosclerosis and recent studies have revealed oxLDL as a key antigen in the development of atherosclerosis. In this study, we assay whether immunizing Apobtm2SgyLdlrtm1Her/J mice with a combination of ApoB and human HSP60 peptides has an additive effect on atheroprotection compared to ApoB or HSP60 peptides applied alone by following atherosclerotic lesion development. Methods and results: In this study, 2 weeks after the first immunization, Apobtm2SgyLdlrtm1Her/J mice were placed on a high-fat diet for 8 weeks followed by 2 weeks on a normal diet allowing the mice to adapt to the environment before sacrifice. High levels of ApoB and HSP60 antibodies were detectable in week 2 and week 12 following the first immunization with KLH-conjugated ApoB and HSP60 peptides either individually or in combination. Histological analyses demonstrated that mice immunized with both, ApoB and HSP60 peptides, showed the most significant reduction in atherosclerotic lesions (41.3%; ptm2SgyLdlrtm1Her/J mouse model of atherosclerosis. This approach offers promise as a novel strategy for developing anti-atherosclerotic agents.

Original languageEnglish
Pages (from-to)472-480
Number of pages9
JournalAtherosclerosis
Volume212
Issue number2
DOIs
Publication statusPublished - Oct 2010

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Apolipoproteins B
Epitopes
Immunization
Atherosclerosis
Peptides
High Fat Diet
Autoantibodies
Diet
Antigens
Antibodies

Keywords

  • ApoB-100
  • HSP60 and atherosclerosis
  • LDLR
  • OxLDL

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Immunization with a combination of ApoB and HSP60 epitopes significantly reduces early atherosclerotic lesion in Apobtm2SgyLdlrtm1Her/J mice. / Lu, Xinjie; Chen, Daxin; Endrész, V.; Xia, Min; Faludi, Ildiko; Burián, K.; Szabo, Andrea; Csanadi, Agnes; Miczák, A.; Gönczöl, E.; Kakkar, Vijay.

In: Atherosclerosis, Vol. 212, No. 2, 10.2010, p. 472-480.

Research output: Contribution to journalArticle

Lu, Xinjie ; Chen, Daxin ; Endrész, V. ; Xia, Min ; Faludi, Ildiko ; Burián, K. ; Szabo, Andrea ; Csanadi, Agnes ; Miczák, A. ; Gönczöl, E. ; Kakkar, Vijay. / Immunization with a combination of ApoB and HSP60 epitopes significantly reduces early atherosclerotic lesion in Apobtm2SgyLdlrtm1Her/J mice. In: Atherosclerosis. 2010 ; Vol. 212, No. 2. pp. 472-480.
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AU - Xia, Min

AU - Faludi, Ildiko

AU - Burián, K.

AU - Szabo, Andrea

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