Immunization of guinea pigs with human choline acetyltransferase induces selective lower motoneuron destruction

J. Engelhardt, L. Siklós, Stanley H. Appel

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Prior studies have demonstrated that guinea pigs immunized with bovine spinal motoneurons develop immune-mediated lower motoneuron disease. In the present experiments, guinea pigs immunized with choline acetyltransferase (ChAT) from human placenta develop lower motoneuron destruction and striated muscle atrophy. In this model, increased IgG was detected in lower motoneurons and at the motor end-plate by immunocytochemistry. Ultrastructural analysis revealed an increase in calcium content and in the density of synaptic vesicles in axon terminals at neuromuscular junctions. Similar morphological changes could be induced in mice following passively transfer of IgG from ChAT-immunized guinea pigs. The increased IgG uptake and raised calcium content in motor axon terminals as well as the selective lower motoneuron damage, suggest that a similar final common pathway can lead to motoneuron injury following immunization with human placental ChAT or bovine spinal motoneurons.

Original languageEnglish
Pages (from-to)57-68
Number of pages12
JournalJournal of Neuroimmunology
Volume78
Issue number1-2
DOIs
Publication statusPublished - Sep 1997

Fingerprint

Choline O-Acetyltransferase
Motor Neurons
Immunization
Guinea Pigs
Immunoglobulin G
Presynaptic Terminals
Motor Endplate
Calcium
Striated Muscle
Muscular Atrophy
Synaptic Vesicles
Neuromuscular Junction
Placenta
Immunohistochemistry
Wounds and Injuries

Keywords

  • Choline acetyltransferase
  • End-plate
  • Guinea pig
  • Immunization
  • Intracellular calcium
  • Motoneuron disease

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

Cite this

Immunization of guinea pigs with human choline acetyltransferase induces selective lower motoneuron destruction. / Engelhardt, J.; Siklós, L.; Appel, Stanley H.

In: Journal of Neuroimmunology, Vol. 78, No. 1-2, 09.1997, p. 57-68.

Research output: Contribution to journalArticle

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