Immune response modulation by targeted complexes based on streptavidin

Zsuzsanna Szekeres, Melinda Herbáth, J. Prechl

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Several immunomodulatory constructs based on streptavidin-biotin binding have been described that exploit the modularity and flexibility of this system. In general, a streptavidin core serves as the carrier of targeting and effector molecules. Targeting agents, such as whole antibodies or their fragments and natural ligands, deliver antigen to specific receptors on leukocytes. This targeted delivery can both enhance and fine-tune immune responses, which are directed against streptavidin itself or its cargo. Due to its tetrameric structure, streptavidin-based targeting complexes can crosslink cell surface receptors. This property enables such constructs to initiate signaling events in the target cells. Thus, in addition to improving antigen uptake, activation of APCs can be achieved. By using liposome- or nanobead-bound streptavidin, one can both boost the crosslinking effect and target different cell populations, specialized in the uptake of particulate antigens. To generate streptavidin-based constructs, various molecular biological and chemical methods have been applied. In a preferred setup, monobiotinylated components are used, in order to ensure the formation of complexes with controllable stoichiometry. This strategy also allows the combination of distinct targeting and antigenic moieties in the same complex. Here we review various approaches of streptavidin-based immunotargeting and their effects on immune responses.

Original languageEnglish
Title of host publicationBiochemistry Research Updates
PublisherNova Science Publishers, Inc.
Pages48-85
Number of pages38
ISBN (Print)9781612097008
Publication statusPublished - 2011

Fingerprint

Streptavidin
Modulation
Antigens
Cell Surface Receptors
Biotin
Liposomes
Stoichiometry
Crosslinking
Leukocytes
Chemical activation
Cells
Ligands
Molecules
Antibodies
Population

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Szekeres, Z., Herbáth, M., & Prechl, J. (2011). Immune response modulation by targeted complexes based on streptavidin. In Biochemistry Research Updates (pp. 48-85). Nova Science Publishers, Inc..

Immune response modulation by targeted complexes based on streptavidin. / Szekeres, Zsuzsanna; Herbáth, Melinda; Prechl, J.

Biochemistry Research Updates. Nova Science Publishers, Inc., 2011. p. 48-85.

Research output: Chapter in Book/Report/Conference proceedingChapter

Szekeres, Z, Herbáth, M & Prechl, J 2011, Immune response modulation by targeted complexes based on streptavidin. in Biochemistry Research Updates. Nova Science Publishers, Inc., pp. 48-85.
Szekeres Z, Herbáth M, Prechl J. Immune response modulation by targeted complexes based on streptavidin. In Biochemistry Research Updates. Nova Science Publishers, Inc. 2011. p. 48-85
Szekeres, Zsuzsanna ; Herbáth, Melinda ; Prechl, J. / Immune response modulation by targeted complexes based on streptavidin. Biochemistry Research Updates. Nova Science Publishers, Inc., 2011. pp. 48-85
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