Immune recognition of human colonic-tumour-associated MUC-2 mucins using an anti-peptide antibody

M. R. Price, M. Sekowski, A. Ladányi, K. Uray, Y. Ma, L. Durrant, S. J B Tendler

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

In human intestinal malignancy, alterations occur in the expression of mucins defined by the MUC-2 gene. These changes include the unmasking of epitopes in the mucin protein core. In order to probe these modifications associated with mucins of the malignant phenotype, a monoclonal antibody (MAb) was developed against synthetic peptide with a sequence based upon that of the protein core of the MUC-2 mucin. The antibody (designated 996) was shown to recognize a high-molecular-weight glycoprotein from colonic carcinoma tissue. The material reacted uniformly with Concanavalin A but variably with other lectins, indicating heterogeneity in the associated oligosaccharide side chains. The protein core was accessible both to 996 antibody binding and to degradation with proteases. Immunization with the affinity-purified mucin-like material elicited antibodies reactive with both the immunogen and the synthetic peptides, confirming the immunogenic character of protein-core determinants. Epitope mapping studies, using synthetic peptides in solution and synthetic peptides tethered to the heads of plastic pins, indicated that the minimum epitope for the 996 antibody is a tetramer of T G T Q. Antibody interaction with the glutamine (Q) residue was determined to be of major importance in the antigen-antibody reaction. The findings illustrate the characterization of an anti-peptide antibody which may be used to probe alterations in MUC-2 mucin expression associated with human intestinal malignant disease.

Original languageEnglish
Pages (from-to)753-759
Number of pages7
JournalInternational Journal of Cancer
Volume55
Issue number5
DOIs
Publication statusPublished - 1993

Fingerprint

Mucin-2
Mucins
Anti-Idiotypic Antibodies
Peptides
Antibodies
Neoplasms
Epitopes
Proteins
Antigen-Antibody Reactions
Epitope Mapping
Intestinal Diseases
Synthetic Vaccines
Concanavalin A
Glutamine
Oligosaccharides
Lectins
Plastics
Immunization
Glycoproteins
Peptide Hydrolases

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Immune recognition of human colonic-tumour-associated MUC-2 mucins using an anti-peptide antibody. / Price, M. R.; Sekowski, M.; Ladányi, A.; Uray, K.; Ma, Y.; Durrant, L.; Tendler, S. J B.

In: International Journal of Cancer, Vol. 55, No. 5, 1993, p. 753-759.

Research output: Contribution to journalArticle

Price, M. R. ; Sekowski, M. ; Ladányi, A. ; Uray, K. ; Ma, Y. ; Durrant, L. ; Tendler, S. J B. / Immune recognition of human colonic-tumour-associated MUC-2 mucins using an anti-peptide antibody. In: International Journal of Cancer. 1993 ; Vol. 55, No. 5. pp. 753-759.
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