Immune complex binding induces lateral lipid re-ordering in rat macrophage plasma membrane

M. Sandor, Cs Bagyinka, L. I. Horváth, G. A. Medgyesi, Kata Miklós, J. Gergely

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The plasma membrane of rat peritoneal exudate cells was spin labelled with fatty acid spin probes and structural changes induced by ligand binding to Fc and C3b receptors were followed. Neither antigens, nor monovalent immune complexes were capable of inducing any change. Multivalent ligands, both in soluble and particulate form, and to a lesser extent uncomplexed immunoglobulins were very effective in inducing membrane re-ordering, i.e. a decrease in fluidity. The phase transition region markedly broadened upon binding of multivalent immune complexes, indicating phase separation. There was a time lag of 5-10 min before the onset of lipid re-ordering was observed. We suggest that multivalent binding that is capable of cross-linking surface receptors initiates lateral phase separation and lipid re-ordering due to receptor aggregation.

Original languageEnglish
Pages (from-to)173-179
Number of pages7
JournalMolecular Immunology
Volume18
Issue number3
DOIs
Publication statusPublished - 1981

Fingerprint

Antigen-Antibody Complex
Receptor Aggregation
Macrophages
Complement 3b Receptors
Cell Membrane
Ligands
Lipids
Fc Receptors
Phase Transition
Exudates and Transudates
Immunoglobulins
Fatty Acids
Antigens
Membranes

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

Cite this

Immune complex binding induces lateral lipid re-ordering in rat macrophage plasma membrane. / Sandor, M.; Bagyinka, Cs; Horváth, L. I.; Medgyesi, G. A.; Miklós, Kata; Gergely, J.

In: Molecular Immunology, Vol. 18, No. 3, 1981, p. 173-179.

Research output: Contribution to journalArticle

Sandor, M. ; Bagyinka, Cs ; Horváth, L. I. ; Medgyesi, G. A. ; Miklós, Kata ; Gergely, J. / Immune complex binding induces lateral lipid re-ordering in rat macrophage plasma membrane. In: Molecular Immunology. 1981 ; Vol. 18, No. 3. pp. 173-179.
@article{e303c6b4d27a4375a139748af1cef152,
title = "Immune complex binding induces lateral lipid re-ordering in rat macrophage plasma membrane",
abstract = "The plasma membrane of rat peritoneal exudate cells was spin labelled with fatty acid spin probes and structural changes induced by ligand binding to Fc and C3b receptors were followed. Neither antigens, nor monovalent immune complexes were capable of inducing any change. Multivalent ligands, both in soluble and particulate form, and to a lesser extent uncomplexed immunoglobulins were very effective in inducing membrane re-ordering, i.e. a decrease in fluidity. The phase transition region markedly broadened upon binding of multivalent immune complexes, indicating phase separation. There was a time lag of 5-10 min before the onset of lipid re-ordering was observed. We suggest that multivalent binding that is capable of cross-linking surface receptors initiates lateral phase separation and lipid re-ordering due to receptor aggregation.",
author = "M. Sandor and Cs Bagyinka and Horv{\'a}th, {L. I.} and Medgyesi, {G. A.} and Kata Mikl{\'o}s and J. Gergely",
year = "1981",
doi = "10.1016/0161-5890(81)90083-3",
language = "English",
volume = "18",
pages = "173--179",
journal = "Molecular Immunology",
issn = "0161-5890",
publisher = "Elsevier Limited",
number = "3",

}

TY - JOUR

T1 - Immune complex binding induces lateral lipid re-ordering in rat macrophage plasma membrane

AU - Sandor, M.

AU - Bagyinka, Cs

AU - Horváth, L. I.

AU - Medgyesi, G. A.

AU - Miklós, Kata

AU - Gergely, J.

PY - 1981

Y1 - 1981

N2 - The plasma membrane of rat peritoneal exudate cells was spin labelled with fatty acid spin probes and structural changes induced by ligand binding to Fc and C3b receptors were followed. Neither antigens, nor monovalent immune complexes were capable of inducing any change. Multivalent ligands, both in soluble and particulate form, and to a lesser extent uncomplexed immunoglobulins were very effective in inducing membrane re-ordering, i.e. a decrease in fluidity. The phase transition region markedly broadened upon binding of multivalent immune complexes, indicating phase separation. There was a time lag of 5-10 min before the onset of lipid re-ordering was observed. We suggest that multivalent binding that is capable of cross-linking surface receptors initiates lateral phase separation and lipid re-ordering due to receptor aggregation.

AB - The plasma membrane of rat peritoneal exudate cells was spin labelled with fatty acid spin probes and structural changes induced by ligand binding to Fc and C3b receptors were followed. Neither antigens, nor monovalent immune complexes were capable of inducing any change. Multivalent ligands, both in soluble and particulate form, and to a lesser extent uncomplexed immunoglobulins were very effective in inducing membrane re-ordering, i.e. a decrease in fluidity. The phase transition region markedly broadened upon binding of multivalent immune complexes, indicating phase separation. There was a time lag of 5-10 min before the onset of lipid re-ordering was observed. We suggest that multivalent binding that is capable of cross-linking surface receptors initiates lateral phase separation and lipid re-ordering due to receptor aggregation.

UR - http://www.scopus.com/inward/record.url?scp=0019351875&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019351875&partnerID=8YFLogxK

U2 - 10.1016/0161-5890(81)90083-3

DO - 10.1016/0161-5890(81)90083-3

M3 - Article

C2 - 6267449

AN - SCOPUS:0019351875

VL - 18

SP - 173

EP - 179

JO - Molecular Immunology

JF - Molecular Immunology

SN - 0161-5890

IS - 3

ER -