Immobilization strategies and electrochemical evaluation of porous silicon based cytochrome c electrode

Jessica Márquez, Luis F. Cházaro-Ruiz, L. Zimányi, Gabriela Palestino

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Quasi-reversible and direct electrochemistry has been observed at a new electrochemical interface constructed by functionalization of porous silicon microcavities (PSiMc) with either amino (APTES) or mercapto (MPTS) propyltriethoxysilane and the redox protein cytochrome c (cyt c). The high specific surface area, tunable pore size (observed by scanning electron microscopy) and functional surface of PSiMc permitted the infiltration of molecular binders and cyt c into the porous structure. The optical response of PSiMc was evaluated during all modification steps. In all the cases a red shift in the reflectance spectra after substrate modification was observed, indicating the infiltration and immobilization of organic binders and the cyt c protein. Dose-response curves demonstrated that biofunctionalization with APTES followed by glutaraldehyde (GTA) amine activation provides higher amounts of immobilized protein with 4.73 nmol protein/cm2 of etching area compared with 1 nmol protein/cm2 when using MPTS as a molecular binder. Infrared spectroscopy (FTIR) also demonstrated the adsorption of the protein within the surface nanostructure. The PSiMc/cyt c hybrid devices showed high electrochemical stability due to the retaining of the protein's redox activity even after several months of its immobilization

Original languageEnglish
Pages (from-to)550-556
Number of pages7
JournalElectrochimica Acta
Volume140
DOIs
Publication statusPublished - Sep 10 2014

Fingerprint

Porous silicon
Cytochromes c
Microcavities
Proteins
Electrodes
Binders
Infiltration
Immobilized Proteins
Glutaral
Electrochemistry
Specific surface area
Pore size
Amines
Infrared spectroscopy
Etching
Nanostructures
Chemical activation
Adsorption
Scanning electron microscopy
Substrates

Keywords

  • bioelectrode
  • biofunctionalization
  • cyclic voltammetry
  • cytochrome c
  • porous silicon

ASJC Scopus subject areas

  • Electrochemistry
  • Chemical Engineering(all)

Cite this

Immobilization strategies and electrochemical evaluation of porous silicon based cytochrome c electrode. / Márquez, Jessica; Cházaro-Ruiz, Luis F.; Zimányi, L.; Palestino, Gabriela.

In: Electrochimica Acta, Vol. 140, 10.09.2014, p. 550-556.

Research output: Contribution to journalArticle

Márquez, Jessica ; Cházaro-Ruiz, Luis F. ; Zimányi, L. ; Palestino, Gabriela. / Immobilization strategies and electrochemical evaluation of porous silicon based cytochrome c electrode. In: Electrochimica Acta. 2014 ; Vol. 140. pp. 550-556.
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